| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PTGER3 recombinant protein (Position: K2-R390) was used as the immunogen for the PTGER3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PTGER3 Antibody / Prostaglandin E2 receptor EP3 is a anti-PTGER3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PTGER3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, PTGER3 is a seven-transmembrane GPCR with extracellular ligand-binding domains and intracellular loops that interact with heterotrimeric G proteins. It belongs to the rhodopsin-like GPCR family, sharing sequence homology with other prostanoid receptors. Co-localization studies show PTGER3 localized on the plasma membrane of smooth muscle cells, immune cells, and neurons. The receptor�s cytoplasmic tail contains regions that determine isoform-specific signaling and regulatory properties.
Functionally, PTGER3 mediates PGE2-dependent inhibition of adenylate cyclase via Gi coupling, leading to decreased intracellular cAMP levels. In smooth muscle, this signaling pathway promotes contraction, contributing to uterine and gastrointestinal motility. In the central nervous system, PTGER3 influences fever generation and pain modulation through hypothalamic pathways. The receptor also regulates renal sodium excretion, vascular tone, and platelet aggregation. Known ligands include PGE2 and selective agonists such as sulprostone and misoprostol.
PTGER3 expression is widespread but particularly high in uterus, kidney, stomach, and brain. Dysregulation of PTGER3 has been associated with inflammatory diseases, reproductive disorders, and cancer. Overexpression contributes to tumor progression by promoting angiogenesis and immune evasion, while inhibition reduces inflammation and hyperalgesia. Pathway associations include prostaglandin signaling, GPCR-mediated calcium signaling, and cAMP regulation. During development, PTGER3 contributes to uterine contractility and thermoregulatory responses.
The PTGER3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
