| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human PTPRE recombinant protein (Position: Q74-H591) was used as the immunogen for the PTPRE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PTPRE Antibody / Protein tyrosine phosphatase epsilon is a anti-PTPRE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Intermediate filaments).
Key elements and design rationale
- Target: PTPRE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, PTPRE negatively regulates receptor tyrosine kinase signaling pathways, including EGFR, PDGFR, and insulin receptor signaling. By dephosphorylating these kinases and associated adaptor proteins, it modulates cellular responses to growth factors and cytokines. PTPRE also participates in the regulation of voltage-gated potassium channels, influencing neuronal excitability and osteoclast function. Co-localization studies show PTPRE interacting with Kv2.1 channels and integrin complexes at the plasma membrane.
Structurally, PTPRE contains a catalytic phosphatase domain with the conserved HCXGR motif responsible for substrate dephosphorylation and a regulatory domain that mediates dimerization and subcellular targeting. The receptor isoform features an extracellular region, a single transmembrane helix, and two intracellular catalytic domains, whereas the cytoplasmic variant lacks the transmembrane segment. PTPRE is classified within the receptor-type PTP family, which includes PTPRA and PTPRJ, sharing high sequence and structural similarity.
PTPRE is essential in regulating bone resorption, neuronal signaling, and immune cell activation. In osteoclasts, it modulates integrin-mediated cytoskeletal organization and bone matrix degradation. In neurons, PTPRE fine-tunes ion channel phosphorylation, controlling electrical activity. It is also involved in macrophage and mast cell activation through modulation of Src family kinases. Known substrates include the EGF receptor, p130Cas, and voltage-gated potassium channels, linking PTPRE to multiple signaling pathways including MAPK and PI3K-AKT.
Dysregulation of PTPRE expression contributes to pathological conditions such as osteoporosis, cancer, and autoimmune disorders. Overexpression has been observed in breast cancer, where it enhances oncogenic signaling and invasion, while loss of function leads to impaired osteoclast activity and bone remodeling defects. Pathway associations include dephosphorylation signaling, Rho GTPase regulation, and integrin signaling networks. Developmentally, PTPRE is expressed in neural crest and mesenchymal cells, supporting differentiation and migration during embryogenesis.
Immunohistochemical staining using PTPRE antibody shows membrane and cytoplasmic localization in neurons, osteoclasts, and epithelial cells. The PTPRE antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
