| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C2orf79/PTRHD1 recombinant protein (Position: M1-K140) was used as the immunogen for the PTRHD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PTRHD1 Antibody / Peptidyl-tRNA hydrolase domain-containing protein 1 is a anti-PTRHD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Miochondria.
Key elements and design rationale
- Target: PTRHD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, PTRHD1 antibody identifies a 220-amino-acid protein that associates with translational complexes and ubiquitin-proteasome pathways. PTRHD1 is proposed to participate in the recycling of prematurely released peptidyl-tRNAs during protein synthesis, preventing accumulation of stalled translation intermediates. It may also regulate proteasomal activity by binding to ubiquitin-like proteins and adaptors such as UBQLN4, thereby influencing protein degradation and quality control under cellular stress conditions.
The PTRHD1 gene is located on chromosome 2p23.3 and is expressed predominantly in brain, testis, and liver. Its expression pattern and domain composition suggest a dual role in neuronal homeostasis and proteostatic regulation. PTRHD1 may serve as a molecular bridge connecting translation fidelity to protein turnover and synaptic function.
Pathologically, biallelic loss-of-function mutations in PTRHD1 cause autosomal recessive early-onset parkinsonism with intellectual disability, highlighting its importance in maintaining neuronal health. Impaired PTRHD1 function results in accumulation of defective translation products and increased cellular stress, leading to neurodegeneration. Research using PTRHD1 antibody supports studies in translational control, proteostasis, and neurogenetic disorders.
PTRHD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect small cytoplasmic regulatory proteins.
Structurally, Peptidyl-tRNA hydrolase domain-containing protein 1 contains an N-terminal hydrolase-like domain similar to bacterial PTH enzymes but lacking key catalytic residues, suggesting a non-enzymatic regulatory role. The protein's C-terminal region supports interactions with ubiquitin-binding proteins. This antibody facilitates exploration of PTRHD1's role in neuronal proteostasis and translation fidelity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
