| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PUDP recombinant protein (Position: M1-E228) was used as the immunogen for the PUDP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PUDP Antibody / Pseudouridine 5-phosphatase is a anti-PUDP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PUDP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
PUDP is a member of the haloacid dehalogenase (HAD) superfamily of hydrolases and shares structural features with sugar phosphatases, including a conserved catalytic Asp residue that facilitates phosphate hydrolysis. The enzyme functions primarily in the cytoplasm and exhibits substrate specificity for pseudouridine monophosphate over canonical nucleotides. PUDP activity supports RNA salvage by clearing modified nucleosides generated from rRNA and tRNA decay.
The PUDP antibody is used in enzymology, RNA metabolism, and nucleotide biochemistry research to study RNA turnover and modified nucleotide processing. Western blot analysis identifies a 31 kilodalton band corresponding to PUDP, while immunofluorescence shows diffuse cytoplasmic localization consistent with its metabolic role. This antibody provides a useful reagent for monitoring nucleotide recycling and evaluating enzymatic regulation in metabolic and stress-response pathways.
Altered expression of PUDP has been associated with cancer, where metabolic reprogramming changes nucleotide flux and RNA stability. By participating in modified nucleotide clearance, PUDP helps maintain nucleotide pool balance and prevents accumulation of potentially toxic intermediates. The PUDP antibody supports studies into RNA degradation, nucleotide recycling, and metabolic adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
