| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PUF60 recombinant protein (Position: Q48-A559) was used as the immunogen for the PUF60 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PUF60 Antibody / Poly(U)-binding splicing factor 60 kDa is a anti-PUF60 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PUF60
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IP, FACS, ELISA
Biological background
Functionally, PUF60 antibody identifies a 548-amino-acid protein containing two RNA recognition motifs (RRMs) that mediate specific binding to pre-mRNA and single-stranded DNA. PUF60 enhances exon definition and alternative splicing by interacting with U2AF65, SF3B1, and other splicing factors. It also cooperates with transcriptional regulators to coordinate RNA processing and gene expression.
The PUF60 gene is located on chromosome 8q24.3 and is ubiquitously expressed, with high levels in brain, testis, and proliferative tissues. PUF60 acts as a bridge between transcription and RNA processing machinery, ensuring accurate and efficient mRNA maturation.
Pathologically, mutations or haploinsufficiency of PUF60 cause developmental syndromes characterized by intellectual disability, short stature, and craniofacial abnormalities. Abnormal PUF60 expression has also been associated with cancer and splicing-related disorders. Research using PUF60 antibody supports studies in RNA metabolism, splicing regulation, and genetic disease mechanisms.
PUF60 antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect splicing factors and RNA-binding proteins.
Structurally, Poly(U)-binding splicing factor 60 kDa contains tandem RRMs and a glycine-rich C-terminal region that facilitate RNA recognition and spliceosome assembly. This antibody enables exploration of PUF60's role in RNA splicing and gene expression control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
