| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PYCRL/PYCR3 recombinant protein (Position: M1-D233) was used as the immunogen for the PYCR3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PYCR3 Antibody / Pyrroline-5-carboxylate reductase 3 is a anti-PYCR3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PYCR3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PYCR3 is encoded by the PYCR3 gene located on human chromosome 8q24.3. The protein is approximately 319 amino acids long and localizes to the mitochondrial matrix, functioning alongside PYCR1 and PYCR2 in the proline biosynthetic pathway. PYCR3 preferentially uses NADH as a cofactor and contributes to redox homeostasis by balancing NAD+/NADH ratios within mitochondria.
The PYCR3 antibody detects a 33 kilodalton band by western blot and shows punctate mitochondrial localization under fluorescence microscopy. Through its regulation of proline metabolism, PYCR3 supports anabolic growth, oxidative stress resistance, and survival under nutrient deprivation. It also influences collagen synthesis and extracellular matrix remodeling by controlling intracellular proline availability.
PYCR3 expression is upregulated in several cancers, including colorectal, lung, and prostate carcinoma, where it promotes tumor cell proliferation and metastasis by maintaining mitochondrial NADH pools. Knockdown of PYCR3 reduces ATP production, increases reactive oxygen species, and triggers apoptosis, demonstrating its role in metabolic flexibility. Outside of cancer, PYCR3 supports fibroblast function and tissue repair by providing proline for collagen biosynthesis.
As a regulator of both amino acid metabolism and mitochondrial redox balance, PYCR3 represents a metabolic control point linking biosynthesis and energy maintenance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
