| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PYHIN1 recombinant protein (Position: K86-H488) was used as the immunogen for the PYHIN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PYHIN1 Antibody / Pyrin and HIN domain-containing protein 1 is a anti-PYHIN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: PYHIN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
PYHIN1 functions primarily as a transcriptional regulator and sensor of foreign or damaged DNA. It can bind double-stranded DNA through its HIN domain and modulate transcription of interferon-stimulated genes. In the nucleus, PYHIN1 interacts with transcriptional co-regulators to influence gene expression linked to antiviral defense and tumor suppression. In the cytoplasm, it contributes to inflammasome regulation and apoptotic signaling. Expression of PYHIN1 is strongly induced by type I interferons and inflammatory cytokines, linking it to host defense and immune surveillance.
The PYHIN1 antibody is used in immunology, oncology, and molecular biology to investigate DNA sensing, innate immune activation, and cell cycle regulation. Western blot analysis identifies a 74 kilodalton band corresponding to PYHIN1, while immunofluorescence shows both nuclear and cytoplasmic staining depending on cell type and activation state. Because PYHIN1 acts at the interface of DNA sensing and transcriptional control, the antibody is a valuable tool for studying interferon signaling and genomic stability mechanisms.
Beyond innate immunity, PYHIN1 plays roles in tumor suppression and apoptosis. Its expression inhibits cell proliferation by interfering with E2F transcriptional activity and promoting p53-dependent growth arrest. Loss of PYHIN1 has been reported in several cancers, suggesting that it functions as a tumor suppressor gene. The PYHIN1 antibody enables researchers to explore these functions and track its expression across different physiological and disease contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
