| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human QKI recombinant protein (Position: M1-N341) was used as the immunogen for the QKI antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
QKI Antibody / Protein Quaking is a anti-QKI Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat, Monkey. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: QKI
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
QKI exists in multiple isoforms�QKI-5, QKI-6, and QKI-7�generated through alternative splicing of the C-terminal region. These isoforms differ in subcellular localization and function: QKI-5 resides predominantly in the nucleus and regulates pre-mRNA splicing, QKI-6 shuttles between the nucleus and cytoplasm to coordinate mRNA export and translation, and QKI-7 localizes mainly in the cytoplasm where it modulates mRNA stability. Collectively, these isoforms maintain neuronal and glial cell homeostasis. Mutations or loss of QKI disrupt oligodendrocyte maturation and cause hypomyelination, while abnormal expression contributes to neurodevelopmental disorders and gliomas.
The QKI antibody is widely used in neuroscience and RNA biology research to study RNA metabolism and myelin formation. Western blot analysis detects bands corresponding to the major QKI isoforms ranging from 38 to 45 kilodaltons. Immunofluorescence with this antibody reveals nuclear and cytoplasmic localization patterns depending on isoform distribution and cell type. In the central nervous system, QKI is expressed in oligodendrocytes, astrocytes, and neurons, where it modulates the expression of myelin-related genes such as MBP and PLP1. This makes the QKI antibody a valuable reagent for investigating myelination, glial cell biology, and post-transcriptional regulation.
Beyond the nervous system, QKI influences epithelial-mesenchymal transition, vascular smooth muscle differentiation, and cardiac development. It has also been implicated in tumor suppression, with reduced expression linked to glioblastoma and colorectal cancer progression. The protein interacts with signaling molecules including STAT1 and AKT, suggesting integration between RNA processing and signal transduction. Researchers employ the QKI antibody to monitor these molecular pathways, exploring how altered RNA regulation drives disease phenotypes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
