| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human QRICH1 recombinant protein (Position: K369-D707) was used as the immunogen for the QRICH1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
QRICH1 Antibody / Glutamine-rich protein 1 is a anti-QRICH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: QRICH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
QRICH1 is encoded by the QRICH1 gene located on human chromosome 3q13.33. The protein is approximately 71 kilodaltons and characterized by an N-terminal glutamine-rich region that supports transcriptional activation and protein-protein interactions. QRICH1 functions downstream of the PERK-eIF2-alpha pathway during the integrated stress response. Under ER stress, phosphorylation of eIF2-alpha suppresses general translation but selectively promotes QRICH1 translation, leading to activation of genes involved in protein folding, lipid metabolism, and apoptosis suppression.
The QRICH1 antibody enables detection of the protein by western blot, where it typically appears as a band around 86-100 kilodaltons. Immunofluorescence and confocal microscopy reveal predominant nuclear localization, consistent with its role as a transcriptional regulator. QRICH1 cooperates with transcriptional coactivators to regulate stress-responsive promoters and contributes to reprogramming during differentiation of osteoblasts, neurons, and epithelial cells. Knockdown experiments demonstrate that QRICH1 deficiency enhances sensitivity to ER stress-induced apoptosis, whereas overexpression supports cell survival by restoring proteostasis.
Recent studies link QRICH1 to human skeletal dysplasia, where loss-of-function mutations impair osteoblast differentiation and bone matrix formation. Additionally, QRICH1 modulates lipid homeostasis and is involved in metabolic regulation under nutrient stress. It may also influence viral infection responses through control of translation reinitiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
