| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human QRSL1 recombinant protein (Position: M1-Q528) was used as the immunogen for the QRSL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
QRSL1 Antibody / Glutamyl-tRNA amidotransferase subunit A is a anti-QRSL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: QRSL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Structurally, QRSL1 is a 477-amino-acid mitochondrial matrix protein of approximately 53 kilodaltons containing a glutaminase domain and an amidotransferase catalytic domain that cooperatively enable amide group transfer from glutamine to tRNA-bound glutamate. It interacts with the GATB and GATC subunits to form a functional enzyme complex anchored to mitochondrial ribosomes. QRSL1 is ubiquitously expressed, with high levels in energy-demanding tissues such as heart, muscle, and brain.
The QRSL1 antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, mitochondrial translation accuracy, and metabolic regulation. Western blot analysis detects a 53 kilodalton band corresponding to QRSL1, while immunofluorescence reveals characteristic mitochondrial localization. This antibody serves as a reliable tool for evaluating mitochondrial translation machinery and enzyme complex formation.
Functionally, QRSL1 ensures accurate decoding of the mitochondrial genetic code by converting incorrectly charged tRNAs into their correct aminoacylated forms. Mutations in QRSL1 are linked to mitochondrial encephalopathy and lactic acidosis due to impaired oxidative phosphorylation and protein synthesis defects. The enzyme also contributes to overall mitochondrial proteostasis by preventing misincorporation of amino acids during translation. The QRSL1 antibody supports mechanistic studies of mitochondrial translation, respiratory chain biogenesis, and genetic disorders caused by tRNA modification defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
