| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Alcoholic beverages etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of ethanol and alcohol metabolism. The assay uses OD580nm (Chemical) for signal readout. Compatible sample input includes Alcoholic beverages etc. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD580nm (Chemical) supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Alcoholic beverages etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.0004 for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Detection range 0.04 – 4% alcohol in 96-well plate assay.
Additional feature notes highlight Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day; Versatility. Assays can be executed in 96-well plates or cuvet. Available format information for this listing includes 500 Tests.
Biological background
This product is centered on measurement of ethanol within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) finds applications in basic research, drug discovery, clinic studies, and winery. Simple, direct, and automation-ready procedures for measuring ethanol concentration are very desirable. BioAssay Systems QuantiChrom™ ethanol assay kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Detection method
Colorimetric (OD 580 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.0004.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify ethanol in alcoholic beverages by OD580 nm (Chemical) readout.
- Compare treatment or phenotype groups using matched alcoholic beverages handling.
- Monitor time-course or pre/post changes in alcoholic beverages across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
We are working with a very small murine blood sample (20 μl) and cannot safely remove more than this for our purposes. Will your assay work with such a small sample? Would this require any special methods?
Serum samples will be diluted 3-fold during deproteination. You have to add water to give 100 μL sample for the assay. The ethanol concentration should be 0.04% or higher in the diluted sample, or (>0.2%) in the initial serum sample, in order to be quantified accurately. Our enzyme based ethanol assay (ECET-100), which is more sensitive, might be a better option for you.
Does glucose interfere with the assay?
Yes, glucose and other compound containing hydroxyl groups (sugars, glycerol, etc.) do interfere with the assay. Samples containing high amounts of glucose should be pretreated with our saccaride removal kit (DSRK-500). Our enzyme based ethanol assay (ECET-100), which is more specific, is the better option for such samples.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Prenatal alcohol exposure and maternal glutamine supplementation alter the mTOR signaling pathway in ovine fetal cerebellum and skeletal muscle
Sawant, O. B., et al. (2020). Prenatal alcohol exposure and maternal glutamine supplementation alter the mTOR signaling pathway in ovine fetal cerebellum and skeletal muscle. Alcohol (Fayetteville, N.Y.), 89, 93-102. Assay: Ethanol in sheep blood.
Impact of Wnt/beta-catenin signaling on ethanol-induced changes in brain endothelial cell permeability
Laksitorini, M. D., et al. (2020). Impact of Wnt/beta-catenin signaling on ethanol-induced changes in brain endothelial cell permeability. Journal of Neurochemistry. Assay: Ethanol in mouse tissue and human cells.
A Major Facilitator Superfamily Peptide Transporter From Fusarium oxysporum Influences Bioethanol Production From Lignocellulosic Material
Nugent, B., Ali, S. S., Mullins, E., & Doohan, F. M. (2019). A Major Facilitator Superfamily Peptide Transporter From Fusarium oxysporum Influences Bioethanol Production From Lignocellulosic Material. Frontiers in microbiology 10:295. Assay: Ethanol in Fungus.
NADPH Oxidase Isoform 2 (NOX2) is involved in drug addiction vulnerability in progeny developmentally exposed to ethanol
Contreras, M. L., de la Fuente-Ortega, E., Vargas-Roberts, S., Munoz, D. C., Goic, C. A., & Haeger, P. A. (2017). NADPH Oxidase Isoform 2 (NOX2) is involved in drug addiction vulnerability in progeny developmentally exposed to ethanol. Frontiers in Neuroscience, 11, 338. Assay: Ethanol in Sprague Dewley rats serum.
Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
Duy, D. L., Suda, Y., & Irie, K. (2017). Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase. PloS one, 12(2), e0172476. Assay: Ethanol in E. coli media.
Raw starch fermentation to ethanol by an industrial distiller’s yeast strain of Saccharomyces cerevisiae expressing glucoamylase and alpha-amylase genes
Kim HR, et al (2011). Raw starch fermentation to ethanol by an industrial distiller’s yeast strain of Saccharomyces cerevisiae expressing glucoamylase and alpha-amylase genes. Biotechnol Lett. 33(8):1643-8. Assay: Ethanol in yeast starch.
Effects of voluntary ethanol consumption on emotional state and stress responsiveness in socially isolated rats
Pisu MG, et al (2011). Effects of voluntary ethanol consumption on emotional state and stress responsiveness in socially isolated rats. Eur Neuropsychopharmacol.21(5):414-25. Assay: Ethanol in rat plasma.
Production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor
Alam MZ,et al (2009). Production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor. J Ind Microbiol Biotechnol. 36(6):801-8. Assay: Ethanol in plant oil-palm.
Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis
Lin, YY et al (2009). Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis. Cell 136(6):1073-84. Assay: Ethanol in yeast culture medium.
Altered pattern of Na,K-ATPase activity and mRNA during chronic alcohol consumption by juvenile and adolescent rats
Wang J, et al (2009). Altered pattern of Na,K-ATPase activity and mRNA during chronic alcohol consumption by juvenile and adolescent rats. Cell Mol Neurobiol. 29(1):69-80. Assay: Ethanol in rat blood.
Flumazenil selectively prevents the increase in alpha(4)-subunit gene expression and an associated change in GABA(A) receptor function induced by ethanol withdrawal
Biggio F, et al (2007). Flumazenil selectively prevents the increase in alpha(4)-subunit gene expression and an associated change in GABA(A) receptor function induced by ethanol withdrawal. J Neurochem. 102(3):657-66. Assay: Ethanol in rat primary neuronal cultures, medium.
Inducible nitric oxide synthase attenuates adrenergic signaling in alcohol fed rats
Khanna D, et al (2007). Inducible nitric oxide synthase attenuates adrenergic signaling in alcohol fed rats. J Cardiovasc Pharmacol. 50(6):692-6. Assay: Ethanol in rat serum.
