| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Cells, serum, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of urea and evaluation of drug effects on urea metabolism. The assay uses OD405nm for signal readout. Compatible sample input includes Cells, serum, and other biological samples. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD405nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Cells, serum, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.025 U/L for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.025 to 250 U/L.
Additional feature notes highlight Fast and convenient. The procedure involves addition of a single working reagent and incubation for 20 min. The assay can be run at room temperature or 37°C; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of leucine aminopeptidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
LEUCINE AMINOPEPTIDASE (LAP) (E.C 3.4.11.1)is an enzyme commonly found in liver cells and intestinal cells that hydrolyzes leucine from the N-terminus of proteins and polypeptides. The expression of LAP increases in damage and immune response, so increased LAP activity in serum samples can indicate potential liver damage, tumorigenesis, or inflammation.BioAssay Systems’ LAP activity assay provides a convenient colorimetric method to measure LAP activity in biological samples. In this assay, LAP hydrolyzes a dye-linked leucine substrate, resulting in a yellow, colored product, measurable at OD405nm. The increase in absorbance at 405 nm (ΔOD) in 20 minutes is directly proportional to the LAP activity.
Detection method
Colorimetric (OD 405 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.025 U/L.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify leucine aminopeptidase in cells, serum by OD405 nm readout.
- Compare treatment or phenotype groups using matched cells, serum handling.
- Monitor time-course or pre/post changes in cells, serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Is leucine aminopeptidase enzyme included in the kit?
The customer will need to provide their own enzyme. Leucine Aminopeptidase, microsomal from porcine kidney (e.g Sigma, Cat# L6007) was used for the development of this kit.
I am using the enzyme you recommended to run this assay (Sigma, Cat# L6007). Did you prepare stock solutions in Assay Buffer or is there a better solvent to use?
We dissolved porcine LAP in 0.5M MgCl2. According to BRENDA Enzyme Database, when using this solvent, porcine LAP should be stable indefinitely within pH 7-9.
When preparing Working Reagent, I noticed turbidity after the addition of substrate to Assay Buffer. Is this normal?
Yes, we have occasionally observed the same phenomenon; however, we found after thorough mixing by pipetting up and down at room temperature, the substrate fully dissolves. There has been no observable effect on the reaction from this. We recommend allowing all reagents to come to room temperature prior to preparing Working Reagent. This should help to limit possible turbidity when preparing the assay.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
