| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological, environment etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of urease activity and evaluation/screen for urease inhibitors. The assay uses OD670nm for signal readout. Compatible sample input includes Biological, environment etc. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: OD670nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, environment etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.003 U/L for interpreting low-signal samples.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified; Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of urease within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
UREASE (Amidohydrolase, EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. (NH2)2)CO + H2)O + CO2) + 2NH3) Many gastrointestinal or urinary tract pathogens produce urease. Thus its activity is a useful diagnostic parameter for the presence of pathogens such as Helicobacter pylori. Urease is found in bacteria, yeast, and higher plants. Urease activity is commonly determined in anaerobes of the bovine rumen, human feces and environmental samples such as soils and phytoplanktons. BioAssay Systems’ Urease Assay Kit provides a very sensitive and convenient means to measure urease activity in a variety of samples including soil. In the assay, urease reacts with urea, resulting in the formation of ammonia, which is determined by the Berthelot method at 670nm. The assay is simple, sensitive, stable and high-throughput adaptable.
Detection method
Colorimetric (OD 670 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.003 U/L.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify urease in biological, environment by OD670 nm readout.
- Compare treatment or phenotype groups using matched biological, environment handling.
- Monitor time-course or pre/post changes in biological, environment across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What could be the reason for very low OD readings?
If the standards have low OD values this could be caused by adding the reagents in the wrong order. It is critical to add reagent A first, mix, and then add reagent B. The color reaction is time and temperature dependent. The 30 minute incubation time is for room temperature (25°C). If the temperature is lower, you have to incubate longer to obtain the same OD values.
Could we use this kit for stool sample?
We have not tested feces samples ourselves and are not aware of any publications using this kit with stool samples, but believe it should work: We would suggest homogenizing the feces sample in a neutral buffer (e.g. PBS), urease is water soluble and will dissolve. Depending on the consistence of the sample, I would suggest starting with a 10:1 ratio of buffer to sample and homogenize the sample, e.g. by sonication. Then filter the sample to remove all larger particulates. After that, if necessary, add a centrifugation step (5 min at 15,000 x g) to remove smaller particulates. Only clear supernatant should be used in the assay.
How can we extract urease from soil samples?
Use 0.5 g soil. Add 0.3 mL of 0.4mm glass beads, 500 µL of buffer. Vortex on high speed for 2 minutes, then centrifuge for 2 min at 12000 rpm, use 100 µL of the clear fluid and incubate with substrate at 37°C for 3 hours.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Joerger RD, et al (2020) Effect of sodium bisulfate amendments on bacterial populations in broiler litter. Poultry Scien
Joerger RD, et al (2020) Effect of sodium bisulfate amendments on bacterial populations in broiler litter. Poultry Science.;99(11):5560-5571. Assay: Urease in chicken litter.
Chinese yellow rice wine processing with reduced ethyl carbamate formation by deleting transcriptional regulator dal80p in saccharomyces cerevisiae
Wei, T., et al (2020). Chinese yellow rice wine processing with reduced ethyl carbamate formation by deleting transcriptional regulator dal80p in saccharomyces cerevisiae. Molecules (Basel, Switzerland), 25(16). Assay: Urease in yeast cells.
Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure
Liu, Q. W., Liu, Q. Y., Li, J. Y., Wei, L., Ren, K. K., Zhang, X. C. & Xin, H. B. (2018). Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure. Stem cell research & therapy, 9(1), 321. Assay: Urea production in human cell culture media.
Constitutive expression of the DUR1, 2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentation
Wu, D (2015). Constitutive expression of the DUR1, 2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentation. FEMS Microbiology Letters 363(1): fnv214. Assay: Urease.
Development of an Agrobacterium-mediated transformation system for the cold-adapted fungi Pseudogymnoascus destructans and P
Zhang, T. et al (2015). Development of an Agrobacterium-mediated transformation system for the cold-adapted fungi Pseudogymnoascus destructans and P. pannorum. Fungal Genetics and Biology 81: 73-81. Assay: urease in fungus.
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