Overview
For rapid, direct determination of D-lactic acid concentrations in food and beverage samples as well as biological samples. The assay uses Visual for signal readout. Compatible sample input includes Wine, beer, milk, etc. Typical stated assay timing is Approximately 15 min.
Key elements and design rationale
- Readout format: Visual supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Wine, beer, milk, etc, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Approximately 15 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-180 mg/L (undiluted) D-lactic acid.
Additional feature notes highlight Convenient. No expensive lab equipment needed; Sample treatment and assay can be performed in under 15 minutes. Available format information for this listing includes 10 Tests.
Biological background
This product is centered on measurement of quantiquik d-lactic acid quick test strips within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
LACTIC ACID, or lactate, is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Furthermore, since D-lactate is a specific indicator of bacteria fermentation, its measurement can be used to determine the freshness of milk, meat and fruit juices. Elevated levels of D-lactate in wine is an indication of lactic acid bacteria contamination. BioAssay Systems QuantiQuik™ D-Lactate Test Strips are based on D-Lactate dehydrogenase catalyzed oxidation of D-lactate in which the formed NADH reduces a chromogenic reagent. The intensity of product color, is directly proportional to D-lactate concentration in the sample.
Detection method
Visual.
Procedures and timing
Stated procedure or timing information: Approximately 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Screen quantiquik d-lactic acid quick test strips in wine, beer, milk using strip-based detection.
- Compare threshold or presence-call outcomes across matched sample groups.
- Monitor routine or field-collected batches with consistent collection timing.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What samples have you tested?
The strips have been tested on red wine, white wine, homogenized whole milk, cultured buttermilk, yogurt, malt mash, beer and lemonade.
My sample turns the strip very dark purple, how can I determine the D-lactate concentration?
Very dark purple indicates that the diluted sample concentration is greater than 180 mg/L. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was diluted 5×, try diluting 21×.
I don’t have access to a pipetteman. How can I accurately measure out my samples?
We offer exact volume transfer pipettes as an accessory. For samples requiring a 21× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100).
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.
I am running sour beer samples and know that there should be high levels of lactate in the samples. However, the strips are very patchy and only have color in the corners. What is wrong?
The enzymes in the strips need to be around pH 7 to work properly. Your sour beer may be too acidic, so please adjust the pH with NaOH or another base until it is pH ~7 and rerun the strips. If the levels are quite high, you may also want to dilute the sample in water which will make the sample closer to pH 7 as well since high levels of lactate “max out” the strip.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Limosilactobacillus balticus sp
Li, F et al (2021). Limosilactobacillus balticus sp. Nov., Limosilactobacillus agrestis sp. Nov., Limosilactobacillus albertensis sp. Nov., Limosilactobacillus rudii sp. Nov. And Limosilactobacillus fastidiosus sp. Nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts. International Journal of Systematic and Evolutionary Microbiology, 71(2). Assay: D-Lactic Acid in limosilactobacillus frementation cultures.
