Overview
For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples. The assay uses Visual for signal readout. Compatible sample input includes Soups, sauces, milk, etc. Typical stated assay timing is Approximately 15 min.
Key elements and design rationale
- Readout format: Visual supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Soups, sauces, milk, etc, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Approximately 15 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-300 mg/L (undiluted) L-Glutamate.
Additional feature notes highlight Convenient. No extra equipment is required; Sample treatment and assay can be performed in under 15 minutes. Available format information for this listing includes 10 Tests.
Biological background
This product is centered on measurement of quantiquik l-glutamate quick test strips within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
GLUTAMATE is an important chemical in general metabolism. It is also widely used as a flavor enhancer in the food industry. Consumption of foods containing monosodium glutamate (MSG) has been known to cause headaches, flushing, sweating, nausea, and more. Glutamate is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, autism, and Alzheimer’s disease. BioAssay Systems QuantiQuik™ L-Glutamate Quick Test Strips are based on Glutamate dehydrogenase catalyzed oxidation of glutamate in which the formed NADH reduces a chromogenic reagent. The intensity of the product color is directly proportional to the glutamate concentration in the sample.
Detection method
Visual.
Procedures and timing
Stated procedure or timing information: Approximately 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Screen quantiquik l-glutamate quick test strips in soups, sauces, milk using strip-based detection.
- Compare threshold or presence-call outcomes across matched sample groups.
- Monitor routine or field-collected batches with consistent collection timing.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What samples have you tested?
The strips have been tested on instant soup broth, restaurant Pho broth, soy sauce, fish sauce, 2% milk, plasma, serum, and urine.
What is the recommended dilution for samples with low levels of Glutamate?
For samples with low levels of Glutamate (e.g. plasma, urine, serum, etc) we recommend diluting samples 2×. To do this, carefully transfer 400 µL of sample to the Sample Development Tube. If a pipetteman is not available, we have 400 µL single use transfer pipettes that can be purchased separately (Cat No. TP400). Please note that samples need to have at least 0.1 – 0.2 mM Glutamate to be detected by the strip.
My sample turns the strip very dark purple, how can I determine the L-glutamate concentration?
Very dark purple indicates that the diluted sample concentration is greater than 2 mM. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was diluted 5×, try diluting 21×.
I don’t have access to a pipetteman. How can I accurately measure out my samples?
We offer exact volume transfer pipettes as an accessory. For samples requiring a 21× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100). For samples requiring a 210× dilution, order the 20 µL and 100 µL transfer pipettes.
The color seen on my strip appears to be a different shade than the color chart on the strip bag. What could be the problem?
Fluorescent lights can have a green cast so it is best to view strips under incandescent or natural lighting for optimal color matching.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
