Overview
For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. The assay uses Visual for signal readout. Compatible sample input includes Serum, plasma, urine, etc. Typical stated assay timing is Approximately 15 min.
Key elements and design rationale
- Readout format: Visual supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, etc, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Approximately 15 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea.
Additional feature notes highlight Convenient. No expensive plate or cuvette readers needed; Sample treatment and assay can be performed in under 15 minutes. Available format information for this listing includes 10 Tests.
Biological background
This product is centered on measurement of quantiquik urea (bun) quick test strips within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea is widely used in the agricultural industry as a fertilizer as well as an animal feed additive. Milk urea is often monitored to determine the amount of protein in an animal’s diet. Additionally, small amounts of urea can be found in many kinds of fermented foods and beverages.BioAssay Systems QuantiQuik™ Urea (BUN) Test Strips are based on Urease catalyzed conversion of urea to ammonium and carbon dioxide. The pH of the reaction medium is monitored by a chromogen and the intensity of the product color is directly proportional to the urea concentration in the sample. The QuantiQuik™ Urea Test strips allow for the rapid and inexpensive quantitative determination of urea or blood urea nitrogen (BUN) and do not require sophisticated laboratory instruments.
Detection method
Visual.
Procedures and timing
Stated procedure or timing information: Approximately 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Screen quantiquik urea (bun) quick test strips in serum, plasma, urine using strip-based detection.
- Compare threshold or presence-call outcomes across matched sample groups.
- Monitor routine or field-collected batches with consistent collection timing.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What samples have you tested?
The strips have been tested on serum, plasma, urine, and milk samples.
My sample turns the strip very dark pink, how can I determine the urea concentration?
Very dark pink indicates that the diluted sample concentration is greater than 1500 mg/L Urea. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was tested undiluted, try diluting 5× or 10×.
I don’t have access to a pipetteman. How can I accurately measure out my samples?
We offer exact volume transfer pipettes as an accessory. For samples requiring a 2× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 31× dilution, order the 300 µL transfer pipettes (TP300) and the 20 µL transfer pipettes (TP20).
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.
The strip did not change color even though I know there is urea present in my sample. What is going on?
If the strip is still yellow, but is known to have urea present in it, this could mean that the sample was diluted too much and no longer can be detected by the strips. Another possibility is that something in the sample is interfering with the strip reaction. Samples that are very acidic may interfere with the strip reaction and should be greatly diluted or avoided if possible.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
FGF23 C-tail improves diabetic nephropathy by attenuating renal fibrosis and inflammation
Zhang, X., Guo, K., Xia, F., Zhao, X., Huang, Z., & Niu, J. (2018). FGF23 C-tail improves diabetic nephropathy by attenuating renal fibrosis and inflammation. BMC biotechnology, 18(1), 33. Assay: Ureain mouse urine.
