| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RAB1A recombinant protein (Position: R74-Q199) was used as the immunogen for the RAB1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAB1A Antibody / Ras-related protein Rab-1A is a anti-RAB1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: RAB1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, RAB1A antibody identifies a 205-amino-acid GTP-binding protein that cycles between active GTP-bound and inactive GDP-bound states. Active RAB1A recruits effector proteins such as p115, GM130, and COPII components to mediate vesicle tethering and fusion at the cis-Golgi membrane. It coordinates early secretory pathway events and maintains ER-to-Golgi transport fidelity, supporting proper protein maturation and trafficking.
The RAB1A gene is located on chromosome 2p14 and is ubiquitously expressed in eukaryotic cells, with particularly high expression in secretory tissues. Its activity is regulated by GEFs (guanine nucleotide exchange factors) and GAPs (GTPase-activating proteins) that control the GTP/GDP cycle and subcellular localization.
Pathologically, RAB1A dysregulation affects autophagy, neurodegeneration, and cancer progression. Overexpression enhances cell proliferation and Golgi reorganization in tumor cells, while impaired RAB1A function contributes to ER stress and defective autophagosome formation. Research using RAB1A antibody supports studies in membrane trafficking, Golgi function, and oncogenic signaling.
RAB1A antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect small GTPases.
Structurally, Ras-related protein Rab-1A contains conserved GTP-binding motifs (GxxxxGK[S/T], DxxG, NKxD, and SAx) and a C-terminal geranylgeranylation site that anchors it to membranes. Its switch regions undergo conformational changes upon GTP binding, enabling interaction with effectors that mediate vesicle transport. This antibody allows exploration of RAB1A's role in ER-Golgi trafficking and its contribution to secretory pathway regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
