| Field | Specification |
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| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B was used as the immunogen for the RAB27B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAB27B Antibody / Ras-related protein Rab-27B is a anti-RAB27B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Golgi, Plasma membrane.
Key elements and design rationale
- Target: RAB27B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS
Biological background
RAB27B is encoded by the RAB27B gene located on human chromosome 18q21.32. The protein is approximately 221 amino acids long and cycles between active GTP-bound and inactive GDP-bound states. RAB27B localizes to secretory vesicles and interacts with effector proteins such as SYTL2, MLPH, and Slac2 family members, facilitating vesicle tethering to the plasma membrane before exocytosis.
The RAB27B antibody detects a 25 kilodalton protein by western blot and shows vesicular punctate staining under confocal microscopy. RAB27B functions in a wide range of cell types, including platelets, endocrine cells, and neurons, where it regulates secretion of serotonin, insulin, and neurotransmitters. In melanocytes, RAB27B cooperates with RAB27A to control melanosome transport and pigment distribution.
Dysregulation of RAB27B has been linked to cancer metastasis, neurodegenerative disease, and immune dysfunction. Overexpression enhances invasive potential in breast and colorectal cancers by promoting secretion of exosomes that modify the tumor microenvironment. Mutations or reduced function lead to granule secretion defects and pigmentation abnormalities.
Because RAB27B regulates exocytosis and vesicle transport, it serves as a model for understanding intracellular trafficking mechanisms and communication between cells.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
