| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RAB34 recombinant protein (Position: M1-P259) was used as the immunogen for the RAB34 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAB34 Antibody / Ras-related protein Rab-34 is a anti-RAB34 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RAB34
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, RAB34 antibody identifies a 259-amino-acid cytosolic protein anchored to the Golgi apparatus through its C-terminal prenylation motif. In its GTP-bound state, RAB34 recruits effector proteins that regulate vesicle budding, movement, and fusion. It plays a key role in positioning lysosomes near the perinuclear region by interacting with the motor protein RILP and the lysosomal GTPase RAB7A. Through this mechanism, RAB34 coordinates lysosomal trafficking and degradation of cellular cargo.
The RAB34 gene is located on chromosome 17q11.2 and is expressed in multiple tissues, including brain, liver, and epithelial cells. Its activity supports vesicular transport processes essential for endosome maturation and Golgi integrity. RAB34 also contributes to the formation of ruffled plasma membranes and macropinosomes, thereby facilitating nutrient uptake and receptor internalization. In the Golgi network, it assists in maintaining cisternal stacking and trafficking of glycoproteins to the plasma membrane.
In cell signaling, RAB34 regulates the transport of signaling receptors such as EGFR and PDGFR, influencing downstream MAPK and AKT pathway activation. Dysregulation of RAB34 expression has been linked to cancer progression, where it affects migration, invasion, and exosome secretion. Elevated RAB34 expression correlates with poor prognosis in several cancers, including glioma, pancreatic carcinoma, and hepatocellular carcinoma, suggesting a role in tumor metastasis and lysosome-mediated proteolysis.
RAB34 antibody is commonly used in cell biology, vesicular transport, and cancer research. It is suitable for immunoblotting, immunofluorescence, and co-localization studies to visualize Golgi and lysosomal compartments. This antibody enables detailed examination of RAB34 function in intracellular trafficking, receptor recycling, and organelle positioning. Its detection aids in exploring the role of RAB GTPases in membrane transport and signal regulation.
Structurally, RAB34 shares the conserved GTP-binding motifs found in the RAS superfamily and undergoes prenylation at its C-terminal CAAX box for membrane anchoring. It cycles between active and inactive states under the control of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
