| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human RAB8A was used as the immunogen for the RAB8A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAB8A Antibody / Ras related protein Rab 8A is a anti-RAB8A Rabbit antibody Recombinant Rabbit Monoclonal clone 29R94 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RAB8A
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29R94, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Ras related protein Rab 8A cycles between an inactive GDP bound state and an active GTP bound state, allowing it to coordinate vesicle budding, transport, and fusion events. Active RAB8A recruits specific effector proteins that drive trafficking of vesicles to the plasma membrane, supporting processes such as neurite outgrowth, epithelial polarization, and secretion. Studies using RAB8A antibody have shown its localization to recycling endosomes and the Golgi network, highlighting its role in maintaining polarized trafficking routes in diverse cell types.
Disruption of RAB8A function is linked to ciliopathies and neurodegenerative conditions. RAB8A regulates ciliary membrane extension and signaling, making it critical for developmental pathways such as Hedgehog signaling. Defective Rab protein regulation has also been implicated in Parkinson disease, where altered endocytic recycling and trafficking impair neuronal health. Detection with RAB8A antibody provides valuable insights into these disease mechanisms and offers a tool for studying how vesicular trafficking contributes to pathophysiology.
RAB8A antibody is applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting detects expression in a range of tissues, while immunofluorescence reveals localization to endosomal compartments and the base of primary cilia. Immunohistochemistry further shows distribution in brain, kidney, and epithelial tissues, correlating with its functional roles. Functional assays using RAB8A antibody can track changes in expression following experimental perturbations of membrane trafficking pathways.
By supplying validated RAB8A antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
