Rabbit Anti-Horse IgG Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000571)

What it means: Polyclonal antibodies are produced by multiple B-cell clones, each recognizing a distinct epitope on the target protein — giving Polyclonal antibodies a broad epitope coverage.

Why it matters:

• Polyclonal antibodies typically deliver stronger signal in Western blot and IHC because multiple epitopes remain accessible even after SDS denaturation or fixation, reducing the risk of a false-negative result.
• Greater tolerance for antigen sequence variation across species makes Polyclonal reagents well-suited for studies that span human, mouse, and rat samples in the same experiment.

How to interpret here: Expect robust, high-sensitivity detection with this Polyclonal antibody. Because multiple clones contribute to the signal, titrate carefully — the optimal concentration may be lower than expected. Lot-to-lot variation is possible; validate each lot against a known-positive control.

What it means: This antibody was raised in Rabbit, producing high-affinity IgG with long CDR3 loops that confer exceptional epitope recognition. Anti-rabbit HRP, fluorochrome, and biotin secondary antibodies are universally available across all major suppliers.

Why it matters:

• The Rabbit host species determines which secondary antibody you need for indirect detection — select an anti-Rabbit IgG secondary matched to your detection label (HRP, fluorochrome, or biotin).
• Rabbit-derived antibodies pair optimally with anti-rabbit secondary antibodies. Avoid using rabbit-derived secondary antibodies in experiments involving rabbit tissue — use species-absorbed secondaries or recombinant secondaries instead.

How to interpret here: Confirm availability of an anti-Rabbit secondary antibody in your lab before ordering. For multiplex IF, pair this Rabbit-raised antibody with antibodies from a different host species to avoid secondary antibody cross-reactivity.

What it means: This antibody has validated reactivity to Horse antigen, confirming it binds the Horse ortholog of the target protein in immunoassay conditions.

Why it matters:

• Reactivity to Horse is confirmed through experimental validation — not merely predicted from sequence homology — ensuring reliable performance in Horse-derived samples such as cell lysates, tissues, or serum.
• Use of this antibody with non-Horse samples requires independent validation; predicted cross-reactivity should always be confirmed empirically.

How to interpret here: Ensure your experimental samples are of Horse origin. If you require reactivity to additional species, contact our team to identify a cross-reactive alternative.

What it means: Alexa Fluor 647 format indicates this antibody carries a specific label enabling direct detection in compatible assay systems.

Why it matters:

• The Alexa Fluor 647 conjugate eliminates the secondary antibody incubation step, shortening your protocol and reducing non-specific background from secondary reagents.
• Directly labeled antibodies like this Alexa Fluor 647 format are particularly advantageous in multiplex panels where multiple primary antibodies from the same host species must be distinguished simultaneously.

How to interpret here: Confirm instrument or substrate compatibility with the Alexa Fluor 647 label. Store per datasheet to preserve label integrity — light-sensitive conjugates require protection from light.

What it means: IgG is the immunoglobulin class and subclass of this antibody, defining its constant-region structure and effector functions.

Why it matters:

• Isotype IgG determines which secondary antibody constant-region specificities are compatible — isotype-specific anti-IgG1 or anti-IgG2a secondaries enable discrimination of co-stained antibodies from the same host species in multiplex experiments.
• For functional assays, isotype identity guides selection of Fc-receptor binding controls and isotype-matched negative controls required for rigorous experimental design.

How to interpret here: Use a negative control of the same isotype (IgG) to assess non-specific binding in your assay. If performing multiplex staining with multiple antibodies from the same species, select isotype-specific secondary antibodies to resolve each IgG signal independently.

What it means: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. indicates the chromatographic method used to isolate this antibody from the production matrix, ensuring a defined purity level.

Why it matters:

• The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. removes bulk serum proteins that could cause non-specific binding or elevated background in downstream assays — a critical quality attribute for immunoassay performance.
• The The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. process is routinely validated and documented by the manufacturer as part of QC release, providing traceability for GLP-adjacent studies.

How to interpret here: Confirm the purity specification on the Certificate of Analysis. For the most demanding applications (single-cell IF, proximity ligation), The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. purity is the recommended minimum standard.

What it means: This antibody has been experimentally validated for 3 applications: ELISA, IF/ICC, Flow Cytometry.

Why it matters:

• ELISA
• IF/ICC
• Flow Cytometry
• Each listed application represents an independently optimized protocol — not a theoretical prediction — giving you confidence that the antibody will perform reliably when used at the recommended conditions for that technique.

How to interpret here: Select the application that matches your assay (ELISA, IF/ICC, Flow Cytometry) and refer to the datasheet for the validated dilution range specific to each technique. Using this antibody outside the listed validated applications requires independent optimization and performance verification.