| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human RalA was used as the immunogen for the RALA antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RALA Antibody / Ras-related protein Ral-A is a anti-RALA Rabbit antibody Recombinant Rabbit Monoclonal clone 31R04 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: RALA
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31R04, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Ras-related protein Ral-A functions downstream of Ral guanine nucleotide exchange factors, which activate it in response to Ras and RalGDS signaling. Active Ral-A interacts with effectors such as Sec5 and Exo84, components of the exocyst complex, to regulate polarized exocytosis and vesicle trafficking. Research with RALA antibody has demonstrated that loss of Ral-A disrupts vesicle docking at the plasma membrane, impairing processes such as neurotransmitter release, insulin secretion, and epithelial polarity. This highlights its importance in fundamental cellular logistics.
In cancer biology, Ral-A has been shown to contribute to tumorigenesis independently of Ras. Research using RALA antibody has revealed that Ral-A promotes anchorage-independent growth, invasion, and metastasis in pancreatic and bladder cancers. Unlike Ral-B, which is more closely linked to cell survival, Ral-A is particularly involved in tumor initiation and vesicle trafficking related to oncogenic signaling. Its role as a Ras effector highlights the complexity of Ras-driven oncogenesis and the potential of targeting Ral pathways for therapy.
Beyond cancer, Ras-related protein Ral-A participates in endocytosis, cytoskeletal dynamics, and mitochondrial fission. Studies with RALA antibody have confirmed its localization to the plasma membrane and intracellular vesicles, supporting its roles in trafficking and morphology. Dysregulation of Ral-A has been implicated in metabolic disease and neurological conditions, expanding its significance across multiple fields of study.
RALA antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects Ral-A expression across tissues, immunohistochemistry highlights tissue-specific expression patterns, and immunofluorescence reveals colocalization with vesicle markers and actin structures. Functional assays with RALA antibody enable researchers to link GTPase activation with trafficking, signaling, and cell behavior.
By providing validated RALA antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
