| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RANBP3 recombinant protein (Position: Q26-Q517) was used as the immunogen for the RANBP3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RANBP3 Antibody / Ran-binding protein 3 is a anti-RANBP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: RANBP3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Functionally, RANBP3 antibody identifies a 607-amino-acid cytoplasmic and nuclear protein that interacts with Ran GTPase, CRM1 (also known as Exportin 1), and other nucleocytoplasmic transport components. RANBP3 facilitates CRM1-dependent export of cargo molecules by stabilizing the export complex in a RanGTP-bound state. It also acts as a cofactor promoting efficient release of exported cargo upon GTP hydrolysis. Through these functions, RANBP3 ensures proper distribution of transcription factors, mRNA, and ribonucleoprotein complexes between cellular compartments.
The RANBP3 gene is located on chromosome 19p13.3 and is ubiquitously expressed across mammalian tissues. It contains an N-terminal nuclear export signal, a Ran-binding domain, and multiple leucine-rich motifs responsible for cargo recognition. RANBP3 participates in nuclear export of Smad proteins, linking it directly to TGF-beta signaling. By facilitating Smad nuclear export, it modulates transcriptional responses to growth factors and cytokines. Its activity is regulated by phosphorylation and nuclear-cytoplasmic shuttling that respond to cell cycle and signaling cues.
In addition to its export functions, RANBP3 maintains genome integrity by controlling the localization of DNA repair factors and replication regulators. It also contributes to ribosome biogenesis and mRNA surveillance by interacting with nuclear export adaptors. Dysregulation of RANBP3 has been associated with tumor progression and antiviral defense, as viruses often hijack CRM1-dependent pathways to export viral RNA and proteins.
RANBP3 antibody is widely used in studies of nucleocytoplasmic transport, gene regulation, and signal transduction. It is suitable for immunoblotting, immunofluorescence, and nuclear fractionation assays to examine RANBP3 distribution and function. This antibody supports research into RNA export, TGF-beta signaling, and nuclear pore dynamics. In disease studies, RANBP3 detection helps elucidate altered nuclear transport mechanisms contributing to cancer and viral infection.
Structurally, RANBP3 contains a pleckstrin homology-like domain and a Ran-binding domain that coordinate GTPase activity and CRM1 interaction. Post-translational modifications such as phosphorylation at specific serine residues regulate its subcellular localization and export efficiency.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
