| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD254; ODF; OPGL; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; sOdf |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 210-309 ofhuman RANKL (NP_003692.1). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyThis gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss.
Validated Applications
| Western Blot (WB) | 500 - 1:1000 |
|---|---|
| Immunofluorescence (IF/ICC) | 50 - 1:200 |
| ELISA (ELISA) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 32 kDa |
| Catalog No. | BS72037 |
| UniProt | O14788 |
| Alternative Names | CD254; ODF; OPGL; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; sOdf |
Immunogen / Antigen
Recombinant fusion protein containing a sequence corresponding to amino acids 210-309 ofhuman RANKL (NP_003692.1).
Reactivity Notes
RANKL polyclonal antibody detects endogenous levels of RANKL protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunofluorescence, ELISA. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant fusion protein containing a sequence corresponding to amino acids 210-309 ofhuman RANKL (NP_003692.1).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
