| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RAPH1 recombinant protein (Position: K37-D1199) was used as the immunogen for the RAPH1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAPH1 Antibody / Ras-associated and pleckstrin homology domains-containing protein 1 is a anti-RAPH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: RAPH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Ras-associated and pleckstrin homology domains-containing protein 1 contains Ras-association and pleckstrin homology domains that mediate binding to activated Ras and phosphoinositides, respectively. These domains anchor RAPH1 to the plasma membrane where it recruits actin regulators such as Ena/VASP proteins to promote filament elongation. The RAPH1 antibody supports localization studies revealing how this scaffold integrates receptor-mediated signaling with actin dynamics. Its enrichment at lamellipodia and adhesion sites underscores its central role in coordinating directional migration.
RAPH1 interacts with the small GTPase Rap1 to influence integrin activation and cell adhesion. By bridging signaling pathways that control cytoskeletal organization, it contributes to epithelial integrity, immune cell motility, and neuronal pathfinding. The RAPH1 antibody allows visualization of these processes, providing insight into mechanisms governing cell migration during wound healing and development. RAPH1 dysfunction can impair motility or lead to aberrant invasion in tumor cells.
Lamellipodin has also been implicated in cancer progression and metastasis. Its overexpression enhances actin polymerization and invasive potential in various cancers including breast and colorectal carcinoma. The RAPH1 antibody supports analysis of these oncogenic roles by enabling quantification of protein levels in tumor tissues. Additionally, RAPH1 participates in mechanotransduction pathways by translating substrate stiffness into cytoskeletal rearrangement, linking extracellular cues to motility responses.
The RAPH1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct membrane and cytoplasmic staining consistent with lamellipodial localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
