| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RARS2 recombinant protein (Position: M1-R560) was used as the immunogen for the RARS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RARS2 Antibody / Arginyl-tRNA synthetase 2 is a anti-RARS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RARS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
RARS2 is encoded by the RARS2 gene located on human chromosome 6q16.1. The protein is approximately 580 amino acids long and localizes to the mitochondrial matrix, where it contributes to the translation of 13 essential oxidative phosphorylation (OXPHOS) subunits encoded by mitochondrial DNA. RARS2 functions as part of the aminoacyl-tRNA synthetase family, ensuring fidelity of tRNA charging for mitochondrial translation.
The RARS2 antibody detects a 65 kilodalton protein by western blot and reveals mitochondrial localization under confocal imaging. RARS2 ensures accurate incorporation of arginine during protein synthesis, maintaining the stoichiometry of respiratory chain complexes. Loss of RARS2 activity disrupts mitochondrial protein translation, leading to decreased OXPHOS efficiency and increased oxidative stress.
Mutations in RARS2 cause pontocerebellar hypoplasia type 6, a neurodegenerative disorder characterized by developmental delay, seizures, and cerebellar atrophy. In cellular models, depletion of RARS2 impairs mitochondrial respiration, ATP production, and neuronal viability. Its regulation is closely tied to mitochondrial biogenesis and stress adaptation.
As an essential enzyme for mitochondrial translation and neural health, RARS2 is a valuable model for understanding mitochondrial gene expression and energy homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
