| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human RAB45 was used as the immunogen for the RASEF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
RASEF Antibody / RAB45 / Ras and EF-hand domain-containing protein is a anti-RASEF Rabbit antibody Recombinant Rabbit Monoclonal clone 30R48 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RASEF
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30R48, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
RASEF antibody is widely applied in cancer research, cell biology, and immunology. RASEF has been identified as a tumor suppressor gene in uveal melanoma, where mutations or promoter hypermethylation reduce its expression. Conversely, altered RASEF activity has been observed in lung and breast cancer, suggesting context dependent functions. By detecting RASEF, researchers can investigate its dual roles in tumor biology and cell signaling.
Western blot assays detect RASEF protein in diverse tissue lysates. Immunohistochemistry maps expression in eye, lung, and immune tissues, while immunofluorescence highlights subcellular localization to endosomes and membranes. These methods provide robust tools for analyzing RASEF biology.
RASEF contributes to immune signaling through regulation of endosomal trafficking and receptor recycling. It may influence T cell activation, antigen presentation, and cytokine signaling by modulating endosomal compartments. Its calcium binding EF hand motifs suggest integration of calcium signaling with vesicle dynamics. By applying RASEF antibody, scientists can examine how small GTPases coordinate trafficking and signaling across biological systems.
Beyond immunity and cancer, RASEF has roles in ocular biology, where it is expressed in retinal tissues and linked to eye disease. It also contributes to cellular homeostasis through regulation of vesicle fusion and cytoskeletal remodeling. These diverse functions make RASEF antibody valuable for both basic science and translational research.
RASEF antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
