| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RASSF8 recombinant protein (Position: E50-V419) was used as the immunogen for the RASSF8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RASSF8 Antibody / Ras association domain-containing protein 8 is a anti-RASSF8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Mitochondria.
Key elements and design rationale
- Target: RASSF8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, RASSF8 antibody identifies a 413-amino-acid protein containing an RA (Ras association) domain and a coiled-coil region that mediates homo- and hetero-oligomerization. RASSF8 localizes to adherens junctions and the plasma membrane, where it interacts with proteins such as E-cadherin, beta-catenin, and SCRIB. Through these interactions, RASSF8 supports cell polarity and epithelial integrity by regulating actin cytoskeleton organization. It may also participate in Ras-related signaling pathways that influence apoptosis and cell migration.
The RASSF8 gene is located on chromosome 12p12.1 and is expressed in epithelial and endothelial tissues, with high expression in lung, colon, and skin. It acts downstream of Ras and Rap1A, functioning as a molecular adaptor to relay signals from membrane GTPases to cytoskeletal and junctional complexes. RASSF8 expression is dynamically regulated during epithelial differentiation and stress responses.
Pathologically, loss or reduced expression of RASSF8 is associated with various cancers, including lung, colorectal, and breast carcinoma. Its downregulation disrupts adherens junctions, promotes epithelial-mesenchymal transition (EMT), and enhances tumor cell invasion. RASSF8 may act as a tumor suppressor by maintaining cell adhesion and suppressing uncontrolled proliferation. Research using RASSF8 antibody supports studies in cancer biology, cell adhesion, and epithelial polarity regulation.
RASSF8 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect cytoskeletal and adhesion-related proteins.
Structurally, Ras association domain-containing protein 8 contains an RA domain that binds small GTPases and a C-terminal coiled-coil domain involved in multimerization and membrane targeting. This architecture allows RASSF8 to integrate Ras-related signaling with cytoskeletal remodeling. This antibody facilitates research into RASSF8's role in tumor suppression, cell adhesion, and cytoskeletal integrity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
