| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Sensitivity |
Product Overview
Sandwich ELISA KitAnti-sperm antibodies are produced by cells of the adaptive immune system and are the cause of approximately 7% of male infertility incidences. They can bind to sperm, inhibiting their movement, stopping recognition and entry into the egg, or targeting sperm for destruction when they reach the female reproductive tract.Over half of men who have undergone a vasectomy develop anti-sperm antibodies.Antisperm antibodies in the form of autoantibodies in male and alloantibodies in female patients dire…
Assay Principle
This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AsAb-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated AsAb-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled AsAb-IgG and unlabeled AsAb-IgG with the antibody. A substrate solution is added to the wells and the color deve…
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | Request Information |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
Related Products
Browse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.
This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is Request Information. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
