| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibronectin;FN;Anastellin;Fn1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Target protein isolated from rat plasma |
| Expression system | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies rat fibronectin (gene Fn1) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Fibronectin (FN1) is a large extracellular matrix glycoprotein that binds integrins, collagen, fibrin and heparin. It exists as a soluble plasma form and as insoluble fibrils in tissues, and it supports cell adhesion, migration, wound healing and embryonic development. Fibronectin is a common matrix marker in studies of fibrosis and tissue repair.
Kit Components
| Anti-Rat Fn1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Rat Fn1 Standard | 2 vials, 10 ng/tube |
| Rat Fn1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <15 pg/mL |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–6.8% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.025; 156 = 0.123; 312 = 0.18; 625 = 0.312; 1250 = 0.546; 2500 = 0.919; 5000 = 1.445; 10000 = 2.036. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.025 | 0.123 | 0.18 | 0.312 | 0.546 | 0.919 | 1.445 | 2.036 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 342 | 1852 | 4326 | 361 | 1834 | 4010 |
| Standard deviation | 23.25 | 85.49 | 190.34 | 29.96 | 102.7 | 216.54 |
| CV (%) | 6.8% | 4.6% | 4.4% | 8.3% | 5.6% | 5.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Jiaxin Wang et al. (2025) Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage-Derived Fn1 via FAK/RhoA/ROCK Signaling. Advanced Science. 10.1002/advs.202510631
Salma Malik et al. (2017) Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways. American Journal of Physiology-Renal Physiology. 10.1152/ajprenal.00393.2016
Xu Wen-Wei et al. (2014) Exendin-4 Alleviates High Glucose-Induced Rat Mesangial Cell Dysfunction through the AMPK Pathway. Cellular Physiology and Biochemistry. 10.1159/000358623
Zhiqiang Sun et al. (2018) Artesunate ameliorates high glucose-induced rat glomerular mesangial cell injury by suppressing the TLR4/NF-κB/NLRP3 inflammasome pathway. Chemico-Biological Interactions. 10.1016/j.cbi.2018.07.011
Meiling Zhuang et al. (2016) Preservation of osteoblasts and BM-MSCs biological properties after consecutive passages with the thermal-liftoff method. RSC Advances. 10.1039/C6RA21946B
Yan Bao et al. (2021) PERK-Dependent Activation of the JAK2/STAT3 Pathway Contributes to High Glucose-Induced Extracellular Matrix Deposition in Renal Tubular Epithelial Cells. International Journal of Endocrinology. 10.1155/2021/8475868
Nanda Gamad et al. (2017) Metformin alleviates bleomycin-induced pulmonary fibrosis in rats: Pharmacological effects and molecular mechanisms. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2017.11.101
Xiaoyan Yu et al. (2021) Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase. Experimental and Therapeutic Medicine. 10.3892/etm.2021.9764
Wang Xin-feng et al. (2023) Calcitriol Suppressed Isoproterenol-induced Proliferation of Cardiac Fibroblasts via Integrin β3/FAK/Akt Pathway. Current Medical Science. 10.1007/s11596-022-2681-6
Chuan Lv et al. (2014) Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway. International Journal of Endocrinology. 10.1155/2014/658589
