| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Serum, Plasma, Other Biological Fluids |
| Sensitivity | |
| Storage | |
| UniProt # |
Assay Principle
Il1b (STJE0005440) is a biomarker protein expressed in rat tissues and biological fluids. Dysregulation of STJE0005440 has been implicated in immune regulation and inflammatory responses, making it a relevant biomarker for investigational studies.
Quantitative measurement of STJE0005440 enables researchers to characterize its abundance in Serum, Plasma and correlate levels with experimental conditions. This kit recognizes Rat IL-1 Beta in samples. No significant cross-reactivity or interference between Rat IL-1 Beta and analogues was observed.
This sandwich ELISA kit provides a validated, reproducible format for STJE0005440 quantification across a defined standard curve range of 31.25~2000pg/mL. The assay supports experimental workflows in Immunology Research research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated 96-well microplate (capture antibody), HRP-conjugated detection antibody, recombinant standard protein, sample/standard diluent, concentrated wash buffer, TMB substrate solution, stop solution, and product manual.
Validated Lot Colorimetric ReadoutPerformance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 31.25~2000pg/mL |
| Sensitivity (LLOD) | 18.75pg/mL |
| Validated Sample Types | Serum, Plasma |
| Total Assay Time | 3.5 hours |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| Reactivity | Rat |
| UniProt Accession | Q63264 |
| Storage | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual |
Assay Procedure Summary
Add samples and standards to pre-coated capture antibody wells and incubate. Wash, add HRP-conjugated detection antibody, and incubate. Wash again, add TMB substrate, and stop the reaction. Read absorbance at 450 nm (reference 540–570 nm). Interpolate sample concentrations from the standard curve using a 4-parameter logistic (4-PL) fit. Total protocol time: approximately 3.5 hours.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJE0005440SpeciesRatUniProtQ63264
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 18.75pg/mL. Quantitative standard curve range: 31.25~2000pg/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
Total protocol time is approximately 3.5 hours of hands-on and incubation time. Pre-warm all reagents and prepare dilution series in advance. Set up a timer for each incubation step to maintain consistent timing across plates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Recommended storage: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
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