| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NT-Pro-BNP; N-BNP |
| Detection Range | |
| Product Type | |
| Sample Type(s) | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Sensitivity | |
| Species |
Assay Principle
N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) is a biomarker protein expressed in rat tissues and biological fluids. It is also referred to as NT-Pro-BNP; N-BNP. Dysregulation of NT-ProBNP has been implicated in neurological function and brain physiology, making it a relevant biomarker for investigational studies.
Quantitative measurement of NT-ProBNP enables researchers to characterize its abundance in Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant and correlate levels with experimental conditions. Studies investigating NT-ProBNP have contributed to the understanding of neurological biomarkers, disease pathogenesis, and therapeutic target validation.
This sandwich ELISA kit provides a validated, reproducible format for NT-ProBNP quantification across a defined standard curve range of 78.1-5000pg/mL. The assay supports experimental workflows in Neuroscience Research research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated 96-well microplate (capture antibody), HRP-conjugated detection antibody, recombinant standard protein, sample/standard diluent, concentrated wash buffer, TMB substrate solution, stop solution, and product manual.
Validated Lot Colorimetric ReadoutPerformance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 78.1-5000pg/mL |
| Sensitivity (LLOD) | 27pg/mL |
| Validated Sample Types | Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| Shelf Life | Traditional: 12 months | Ready-to-use: 16 months |
Assay Procedure Summary
Add samples and standards to pre-coated capture antibody wells and incubate. Wash, add HRP-conjugated detection antibody, and incubate. Wash again, add TMB substrate, and stop the reaction. Read absorbance at 450 nm (reference 540–570 nm). Interpolate sample concentrations from the standard curve using a 4-parameter logistic (4-PL) fit.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorDLdevelopCatalog No.DL-NT-ProBNP-Ra, DLR-NT-ProBNP-RaSpeciesRat
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 27pg/mL. Quantitative standard curve range: 78.1-5000pg/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
A typical sandwich ELISA protocol for this kit requires 3.5–5 hours. Pre-warm reagents to room temperature (18–25 °C) for at least 30 minutes before starting and prepare standard dilutions while the capture plate equilibrates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Store the unopened kit at 2–8 °C until the expiry date. Shelf life: Traditional: 12 months | Ready-to-use: 16 months from date of manufacture. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
