| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Rat Primary Rectal Smooth Muscle Cells are rat frozen primary cells from intestine used for vascular remodeling, contractile signaling, and smooth muscle phenotypic regulation studies. These cells display adherent, polygonal growth characteristics.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Intestine
- Growth properties: Adherent, polygonal
- Format: Frozen
- Donor history: Normal tissue
- Biosafety level: II
- Culture context: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4863) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Smooth muscle cells regulate vessel tone, extracellular matrix remodeling, and responses to growth factors, cytokines, and mechanical cues. In culture, they are often used to study contractile-to-synthetic state transitions.
Research relevance and current trends
- Studies frequently examine how inflammatory, mechanical, or metabolic cues alter smooth muscle phenotype and matrix production.
- Primary vascular smooth muscle cells are used to model remodeling pathways relevant to stenosis, calcification, and vascular injury.
- Comparative donor and vessel-source studies help capture regional heterogeneity across the arterial tree.
Common research applications
- Measure contractile marker expression or phenotype switching after defined perturbations.
- Evaluate proliferation, migration, and matrix-remodeling responses in vascular remodeling models.
- Use in co-culture with endothelial cells or fibroblasts to examine vessel wall signaling.
Product-specific data supplied for this listing
- Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4863) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
