| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; Vegfa; Vegf |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A27-R190 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This single-incubation sandwich ELISA quantifies rat vascular endothelial growth factor A (gene Vegfa) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). The strips are coated with an anti-tag antibody. Samples and standards are added to the wells together with the kit’s antibody cocktail (Antibody A and Antibody B); the resulting antibody–target complex is held on the plate through the tag. After washing, TMB substrate is added and the HRP label on the detection antibody converts it to a colored product in proportion to the bound target. The reaction is stopped and the plate is read at 450 nm. The supplier markets the Quick format as a 90-minute assay.
VEGF-A is the main vascular endothelial growth factor that drives angiogenesis. Produced in response to hypoxia, it binds VEGFR1 and VEGFR2 on endothelial cells to promote their proliferation, migration and survival and to increase vascular permeability. VEGF-A is widely studied in vascular development, wound healing, eye disease and tumor angiogenesis.
Kit Components
| Anti-tag Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Rat Vegfa Standard | 2 × 10 ng/tube |
| Rat Vegfa Antibody A | 3 mL |
| Rat Vegfa Antibody B | 3 mL |
| Sample Diluent | 15 mL |
| TBS-T Wash Buffer (25x) | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Adhesive Plate Sealers | 2 |
Also required (not supplied): microplate reader for 450 nm; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution; horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3 mm.
Kit components are not sold separately.
Performance Data
| Species | Rat |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant rat Vegfa (Ala27–Arg190), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.6–8.1% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.043; 15.6 = 0.108; 31.2 = 0.159; 62.5 = 0.260; 125 = 0.443; 250 = 0.795; 500 = 1.396; 1,000 = 2.276. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months. Ships on gel ice and tolerates up to 3 days at room temperature; refrigerate on arrival.
Safety
Sample data
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.043 | 0.108 | 0.159 | 0.260 | 0.443 | 0.795 | 1.396 | 2.276 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 25 | 233 | 364 | 36 | 241 | 398 |
| Standard deviation | 1.4 | 18.87 | 22.93 | 2.45 | 19.04 | 21.89 |
| CV (%) | 5.6% | 8.1% | 6.3% | 6.8% | 7.9% | 5.5% |
Kit components
Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|PieceMaterials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Yongyuan Kang et al. (2025) Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation. Advanced Functional Materials. 10.1002/adfm.202413678
Jintao Zhong et al. (2024) A self-forming bone membrane generated by periosteum-derived stem cell spheroids enhances the repair of bone defects. Acta Biomaterialia. 10.1016/j.actbio.2024.12.058
Jiante Li et al. (2025) Natural origin microfiber scaffolds from microfluidic spinning for gastric tumor postsurgical treatment. Materials & Design. 10.1016/j.matdes.2025.115124
Zhu Yaqiong et al. (2024) Harnessing three-dimensional porous chitosan microsphere embedded with adipose-derived stem cells to promote nerve regeneration. Stem Cell Research & Therapy. 10.1186/s13287-024-03753-w
Yunting Zhang et al. (2025) An adhesive and self-healing ROS-scavenging hydrogel loading with hMSC-derived exosomes for diabetic wound healing. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2025.125315
Runxue Zhou et al. (2023) Bi-functional KIT-PR1P peptides combine with VEGF to protect ischemic kidney in rats by targeting to Kim-1. Regenerative Therapy. 10.1016/j.reth.2023.12.014
Ye X et al. (2022) Effect of diabetes blood-stasis syndrome and Xuefu Zhuyu decoction on ERK1/2-VEGF signal pathway in rat retina Müller cells. Histology and Histopathology. 10.14670/hh-18-427
Eldourghamy Ayman et al. (2023) Naringenin suppresses NLRP3 inflammasome activation via the mRNA-208a signaling pathway in isoproterenol-induced myocardial infarction. Asian Pacific Journal of Tropical Biomedicine. 10.4103/2221-1691.387750
Na Wang et al. (2023) Astragalin attenuates diabetic cataracts via inhibiting aldose reductase activity in rats. International Journal of Ophthalmology. 10.18240/ijo.2023.08.02
