| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Retinoblastoma recombinant protein (Position: E40-D921) was used as the immunogen for the RB1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RB1 Antibody / Retinoblastoma is a anti-RB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: RB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
RB1 is encoded by the RB1 gene located on human chromosome 13q14.2. The protein is approximately 110 kilodaltons and composed of an N-terminal domain, a central pocket domain responsible for E2F binding, and a C-terminal regulatory region. RB1 activity is primarily modulated through phosphorylation by cyclin-dependent kinases (CDKs). Hypophosphorylated RB1 binds and inhibits E2F transcription factors, repressing transcription of S-phase genes. Upon phosphorylation, RB1 releases E2Fs, allowing cell cycle progression.
The RB1 antibody detects the 110 kilodalton protein and phospho-variants by western blot and demonstrates nuclear localization in immunocytochemistry. RB1 serves as a platform for chromatin modifiers, recruiting histone deacetylases (HDACs) and methyltransferases to silence gene expression during quiescence and differentiation. In addition to cell cycle control, RB1 promotes genomic stability by coordinating DNA repair, replication fidelity, and mitotic checkpoints.
Loss or mutation of RB1 is a hallmark of numerous cancers, including retinoblastoma, osteosarcoma, and small-cell lung carcinoma. RB1 inactivation leads to unchecked E2F activity, excessive proliferation, and impaired DNA repair. Conversely, reactivation of RB1 signaling can suppress tumor growth and restore differentiation potential in malignant cells.
Beyond oncology, RB1 regulates tissue-specific differentiation, especially in muscle and neuronal lineages, by interacting with lineage-determining transcription factors. Its phosphorylation status serves as an indicator of cell cycle activity and is frequently used as a diagnostic biomarker.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
