| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RBCK1 recombinant protein (Position: M1-R446) was used as the immunogen for the RBCK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RBCK1 Antibody / RanBP-type and C3HC4-type zinc finger-containing protein 1 is a anti-RBCK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: RBCK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
RBCK1 is encoded by the RBCK1 gene located on human chromosome 20q11.22. The protein contains several domains, including an N-terminal ubiquitin-like (UBL) domain, a RanBP-type zinc finger, a central RBR domain, and a C-terminal NZF-type zinc finger. These motifs enable RBCK1 to interact with ubiquitin, E2 conjugating enzymes, and adaptor proteins to assemble linear and lysine-63-linked ubiquitin chains. Through these modifications, RBCK1 promotes activation of NF-kappaB signaling and contributes to the regulation of cytokine production and cell survival following immune receptor stimulation.
The RBCK1 antibody is useful for detecting the 56-60 kilodalton protein by western blot and immunoprecipitation. In cells, RBCK1 localizes to the cytoplasm and occasionally to the nucleus, depending on signaling state. Functional studies have shown that deficiency or mutation of RBCK1 leads to defects in LUBAC function, resulting in impaired immune signaling, chronic inflammation, or autoinflammatory disorders such as polyglucosan body myopathy. Loss of RBCK1 function also compromises the stability of HOIP, reducing linear ubiquitination of NEMO and other substrates critical for inflammatory gene transcription.
Beyond immunity, RBCK1 participates in protein quality control through the degradation of misfolded cytosolic proteins. It contributes to the ubiquitin-proteasome system and has been implicated in muscle physiology and mitochondrial regulation. Recent studies suggest that RBCK1 also influences cellular metabolism and redox balance, linking ubiquitin signaling to broader physiological processes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
