| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RBM17 recombinant protein (Position: M1-V401) was used as the immunogen for the RBM17 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RBM17 Antibody / RNA-binding motif protein 17 / Splicing factor 45 is a anti-RBM17 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: RBM17
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
RBM17 is a core component of the exon junction complex and is involved in both constitutive and alternative splicing. It modulates inclusion of alternative exons in transcripts related to apoptosis, signaling, and cytoskeletal organization. Knockdown of RBM17 leads to widespread splicing defects and altered expression of cell-cycle genes such as cyclin D1 and BCL2. Elevated expression of RBM17 has been documented in cancers including glioma and breast carcinoma, where it contributes to enhanced proliferation and resistance to apoptosis. Because of this, RBM17 is emerging as a potential therapeutic target in oncology.
The RBM17 antibody is used in molecular biology and cancer research to detect expression and subcellular localization of this splicing regulator. Immunofluorescence studies reveal nuclear speckle localization consistent with active splicing sites, while western blotting identifies a ~55 kilodalton band. The antibody is effective in studies investigating RNA processing, transcriptome regulation, and splicing factor dynamics. Its use in chromatin immunoprecipitation combined with RNA sequencing (CLIP-seq) helps identify RBM17-binding targets genome-wide.
Functionally, RBM17 interacts with RNA helicases and spliceosomal scaffolds to ensure precise splicing during rapid transcriptional responses. In neurons, RBM17 supports maturation of transcripts necessary for synapse formation and plasticity. Dysregulation contributes to developmental disorders and neurodegeneration. The RBM17 antibody therefore provides a versatile tool for exploring RNA metabolism and gene expression regulation at both molecular and cellular levels.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
