| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RBM42 recombinant protein (Position: E73-K469) was used as the immunogen for the RBM42 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RBM42 Antibody / RNA-binding motif protein 42 is a anti-RBM42 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: RBM42
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Structurally, RBM42 contains two conserved RRM domains that enable specific binding to U-rich RNA sequences. It interacts with the spliceosome and ribonucleoprotein complexes to regulate pre-mRNA processing. During cellular stress, RBM42 localizes to stress granules and contributes to the sequestration of mRNAs, preventing unwanted translation and preserving RNA integrity until conditions normalize. It also associates with components of the nonsense-mediated decay pathway, suggesting a role in RNA surveillance and turnover.
The RBM42 antibody is widely used in molecular biology, RNA processing, and stress signaling research to study post-transcriptional control mechanisms and RNA-protein complex dynamics. Western blot analysis identifies a 45 kilodalton band corresponding to RBM42, while immunofluorescence reveals strong nuclear staining with perinucleolar enrichment under basal conditions. Upon stress induction, cytoplasmic granule localization becomes prominent, consistent with its role in RNA regulation and cellular adaptation.
RBM42 is implicated in diverse biological processes, including embryonic development, hematopoiesis, and tumor progression. Its expression is upregulated under hypoxia and oxidative stress, contributing to cellular resistance mechanisms. Dysregulation of RBM42 has been associated with aberrant mRNA metabolism in cancers and neurodegenerative diseases, where mis-splicing and altered RNA transport disrupt homeostasis. The RBM42 antibody enables researchers to track these dynamic changes and characterize its role in RNA fate determination.
Functionally, RBM42 acts as a molecular chaperone for specific mRNAs, maintaining their stability and coordinating translation with cellular energy states. It interfaces with the ribosomal machinery and modulates translation initiation through mRNA secondary structure binding. The RBM42 antibody provides a powerful reagent for exploring RNA biology, splicing regulation, and cellular stress adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
