| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RBPMS2 recombinant protein (Position: K116-Q201) was used as the immunogen for the RBPMS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RBPMS2 Antibody / RNA-binding protein with multiple splicing 2 is a anti-RBPMS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: RBPMS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, RBPMS2 is a ~22 kDa protein with a single RNA recognition motif and a glycine-rich C-terminal region. It localizes to the nucleus and cytoplasm, reflecting dual roles in RNA splicing and translational control. The protein is expressed in the gastrointestinal tract, heart, and developing muscle tissues.
Functionally, RBPMS2 regulates alternative splicing of muscle-specific transcripts, supporting differentiation and contractile function. It also stabilizes RNAs involved in muscle signaling pathways and cytoskeletal dynamics. Researchers use RBPMS2 antibody to study RNA regulation, muscle differentiation, and developmental processes.
Clinically, RBPMS2 expression has been implicated in gastrointestinal diseases, cancer, and heart development. Abnormal expression may contribute to smooth muscle dysfunction and tumorigenesis. In zebrafish, RBPMS2 knockdown disrupts heart development, underscoring its conserved role in cardiac biology.
Experimentally, RBPMS2 antibody is used in western blotting to detect the ~22 kDa protein, in immunofluorescence microscopy to study nuclear-cytoplasmic distribution, and in immunohistochemistry to evaluate tissue expression. RNA immunoprecipitation with RBPMS2 antibody identifies bound transcripts regulating muscle differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
