| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RECK recombinant protein (Position: Q179-K806) was used as the immunogen for the RECK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RECK Antibody / Reversion-inducing cysteine-rich protein with Kazal motifs is a anti-RECK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RECK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, RECK antibody identifies a 971-amino-acid glycosylphosphatidylinositol (GPI)-anchored protein expressed on the cell surface. RECK directly inhibits MMP2, MMP9, and MMP14, limiting ECM degradation and tumor cell invasion. It also stabilizes tissue inhibitors of metalloproteinases (TIMPs) and modulates Notch and Wnt signaling pathways involved in development and tumor suppression. RECK expression is tightly controlled by transcriptional regulators and epigenetic mechanisms, including promoter methylation and microRNA repression.
The RECK gene is located on chromosome 9p13.3 and is broadly expressed in embryonic and adult tissues, including brain, lung, and vascular endothelium. High expression is associated with normal tissue homeostasis and morphogenesis, whereas silencing occurs in many cancers, correlating with poor prognosis.
Pathologically, RECK downregulation promotes cancer metastasis, vascular invasion, and inflammation by unleashing matrix-degrading enzymes. Conversely, forced expression of RECK inhibits tumor angiogenesis and cell migration. RECK deficiency also impairs embryonic vascular development, reflecting its essential role in morphogenesis. Research using RECK antibody supports studies in oncology, extracellular matrix biology, and developmental signaling.
RECK antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect MMP regulators.
Structurally, Reversion-inducing cysteine-rich protein with Kazal motifs contains multiple cysteine-rich and Kazal-type serine protease inhibitor domains that confer high affinity for MMP catalytic sites. Its GPI anchor tethers it to the plasma membrane, positioning RECK for local ECM modulation. This antibody enables exploration of RECK's tumor-suppressive mechanisms and its role in cell-matrix interactions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
