| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Host | |
| Immunogen | A synthetic peptide of human eIF4EBP1 |
| Isotype | |
| Product Type | |
| Shipping | |
| Storage | |
| UniProt # |
Product Overview
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1),is a member of 4EBPs family,which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E,thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors,hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1,alpha,beta and gamma.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:1000 |
| IHC | 1:50-1:100 |
Antibody Specifications
| Target | 4E BP1 |
|---|---|
| Host Species | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Clone Number | R06-8E6 |
| Purification | Affinity Purified |
| Concentration | 300 μg/mL |
| Molecular Weight | Calculated 13kDa; Observed 15/20kDa |
Immunogen
A synthetic peptide of human eIF4EBP1 UniProt: Q13541.
Species Reactivity & Cross-Reactivity
Validated for: Human, Mouse, Rat, Hamster. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Related Products
Browse additional antibodies targeting 4E BP1 and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
The recommended starting dilution for Western Blot is WB 1:500-1:1000;IHC 1:50-1:100. Optimal dilution should be determined empirically for each laboratory setup, sample type, and detection system. Use freshly prepared lysates with protease inhibitors; add phosphatase inhibitors if studying phosphorylation-dependent forms of 4E BP1. Expected band size: Calculated 13kDa, Observed 15/20kDa. If background is high, increase blocking (5% non-fat milk or BSA in TBST) and/or increase wash stringency.
For IHC-P, heat-induced epitope retrieval (HIER) is typically required. Recommended starting conditions: citrate buffer pH 6.0 (microwave or pressure cooker, ~20 min) for rodent samples; EDTA buffer pH 9.0 for human clinical samples. After retrieval, allow sections to cool gradually before proceeding to blocking. Include a 4E BP1-positive tissue control (confirmed by literature or database expression data) and a negative isotype control in every run. Recommended dilution: WB 1:500-1:1000;IHC 1:50-1:100. Refer to the product datasheet for any target-specific retrieval guidance.
This antibody has been experimentally validated in: Human, Mouse, Rat, Hamster. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "A synthetic peptide of human eIF4EBP1" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Storage Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone R06-8E6) provides highly consistent, epitope-specific recognition of 4E BP1, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone R06-8E6) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
