| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Host | |
| Immunogen | A synthetic peptide of human EGFR |
| Isotype | |
| Product Type | |
| Shipping | |
| Storage | |
| UniProt # |
Product Overview
EGFR,also named as ERBB1,is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12,encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression,including cell proliferation,apoptosis,angiogenesis,and metastatic spread. Mutations in this gene are associated with lung cancer.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:2000 |
| IF | 1:50-1:200 |
Antibody Specifications
| Target | EGFR |
|---|---|
| Host Species | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Clone Number | R03-6B1 |
| Purification | Affinity Purified |
| Concentration | 300 μg/mL |
| Molecular Weight | Calculated 134kDa; Observed 175kDa |
Immunogen
A synthetic peptide of human EGFR UniProt: P00533.
Species Reactivity & Cross-Reactivity
Validated for: Human, Mouse. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Related Products
Browse additional antibodies targeting EGFR and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
The recommended starting dilution for Western Blot is WB 1:1000-1:2000;IF 1:50-1:200. Optimal dilution should be determined empirically for each laboratory setup, sample type, and detection system. Use freshly prepared lysates with protease inhibitors; add phosphatase inhibitors if studying phosphorylation-dependent forms of EGFR. Expected band size: Calculated 134kDa, Observed 175kDa. If background is high, increase blocking (5% non-fat milk or BSA in TBST) and/or increase wash stringency.
For intracellular targets including most nuclear and cytoplasmic proteins, fix cells with 4% paraformaldehyde (15 min, room temperature) followed by permeabilization with 0.1–0.2% Triton X-100 or saponin (5–10 min). For surface membrane targets, fixation without permeabilization may be sufficient. Block with 5–10% normal serum (matching the secondary antibody host species) for 30–60 min. Incubate primary antibody at the recommended dilution (WB 1:1000-1:2000;IF 1:50-1:200) at 4°C overnight or 1–2 h at room temperature. Confirm nuclear/cytoplasmic localization of EGFR against published protein databases before designing your protocol.
This antibody has been experimentally validated in: Human, Mouse. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "A synthetic peptide of human EGFR" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Storage Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone R03-6B1) provides highly consistent, epitope-specific recognition of EGFR, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone R03-6B1) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
