| Field | Specification |
|---|---|
| Alternative Names | 4-1BB, CD137-Ig, CD137-huIg, 41BB |
| Conjugate | |
| Formulation | |
| Host | |
| Product Type | |
| Shipping | |
| Storage |
Scientific Background
Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).
Product Description
CHO CellsRecombinant hCD137(4-1BB)-huIg is a recombinant human fusion protein produced in CHO Cells cells. Molecular Structure: A soluble 55 kd fusion protein consisting of the extracellular (186aa) domain of human CD137 fused to human IgG1 Fc (4), with a predicted monomeric molecular weight of 44.1 kd.
Alternative names: 4-1BB, CD137-Ig, CD137-huIg, 41BB
Protein Specifications
| Fusion Format | Human IgG Fc Fusion (huIg) |
|---|---|
| Expression System | CHO Cells |
| Molecular Weight | 55 kDa |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Species Reactivity | Human |
| Validated Applications | ELISA, Flow Cytometry |
| Available Conjugates | Biotin, FITC, PE, Unconjugated |
✔ Research Use Only (RUO)
Functional Activity
CD137 Ig fusion protein blocks binding of anti-human CD137 to activated CEM human tumor cells and also binds to CD137 Ligand on Raji cells.
Safety & Handling
Research Use Only. Not intended for diagnostic, therapeutic, or clinical use. Handle according to good laboratory practice (GLP). Consult the Safety Data Sheet (SDS) prior to use.
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This protein was expressed in CHO Cells cells. Mammalian expression is critical for this Fc fusion protein because it ensures proper glycosylation and tertiary folding of the extracellular immunoglobulin-like domains, preserving native receptor-binding activity.
Recombinant hCD137(4-1BB)-huIg has been validated for: ELISA, Flow Cytometry. It is produced as a Human IgG Fc Fusion (huIg), making it suitable as a blocking reagent, binding standard, or functional stimulant/inhibitor in immunology assays.
Recommended storage: Store at 2 - 5°C. Freeze/Thawing is not recommended.. Formulation: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate. Avoid repeated freeze-thaw cycles. If long-term storage is needed, aliquot upon first use.
Species reactivity includes: human. Cross-reactivity was confirmed by functional binding assays (e.g., ELISA and/or FACS) against cells or recombinant proteins from the indicated species.
Available formats include: Biotin, FITC, PE, Unconjugated. Conjugated forms are ready-to-use without secondary detection reagents for flow cytometry. Unconjugated (purified) forms can be used with anti-Fc secondary antibodies in ELISA.
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- M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227.
- H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404.
- B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98.
- H. Schwarz, et al, (1996) Blood 87: 2839-2845.
- J. Langstein, et al, (1998) J Immunol 160: 2488-2494.
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