| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TALL-1, Blys, THANK, B-cell Activating Factor, TNFSFL13 |
| Conjugate | |
| Formulation | |
| Host | |
| Product Type | |
| Shipping | |
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Scientific Background
The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2 BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4 Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells 1. Cell surface BAFF can be cleaved to form a soluble trimeric molecule.2
Product Description
CHO CellsRecombinant hCD257(BAFF)(trn)-muCD8 is a recombinant human fusion protein produced in CHO Cells cells. Molecular Structure: A soluble molecule consisting of 151 (VQGPEET…c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF was truncated to amino acid 135 to eliminate a potential protease cleavage site.
Alternative names: TALL-1, Blys, THANK, B-cell Activating Factor, TNFSFL13
Protein Specifications
| Fusion Format | Murine CD8α Fusion (muCD8) |
|---|---|
| Expression System | CHO Cells |
| Molecular Weight | 38.5 kDa |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Species Reactivity | Human |
| Validated Applications | ELISA, Flow Cytometry |
| Available Conjugates | Biotin, Unconjugated |
✔ Research Use Only (RUO)
Functional Activity
Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA (8, 9,11).
Safety & Handling
Research Use Only. Not intended for diagnostic, therapeutic, or clinical use. Handle according to good laboratory practice (GLP). Consult the Safety Data Sheet (SDS) prior to use.
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This protein was expressed in CHO Cells cells. Mammalian expression is critical for this Fc fusion protein because it ensures proper glycosylation and tertiary folding of the extracellular immunoglobulin-like domains, preserving native receptor-binding activity.
Recombinant hCD257(BAFF)(trn)-muCD8 has been validated for: ELISA, Flow Cytometry. It is produced as a Murine CD8α Fusion (muCD8), making it suitable as a blocking reagent, binding standard, or functional stimulant/inhibitor in immunology assays.
Recommended storage: Store at 2 - 5°C. Freeze/Thawing is not recommended.. Formulation: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate. Avoid repeated freeze-thaw cycles. If long-term storage is needed, aliquot upon first use.
Species reactivity includes: human. Cross-reactivity was confirmed by functional binding assays (e.g., ELISA and/or FACS) against cells or recombinant proteins from the indicated species.
Available formats include: Biotin, Unconjugated. Conjugated forms are ready-to-use without secondary detection reagents for flow cytometry. Unconjugated (purified) forms can be used with anti-Fc secondary antibodies in ELISA.
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- Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756.
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