| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD269-muIg, CD269-Fc, BCMA-Fc, BCMA-muIg |
| Conjugate | |
| Formulation | |
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| Product Type | |
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Scientific Background
The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2 BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4 Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells1. Recently a third receptor for BAFF (BAFF-R) was described.5 Cell surface BAFF can be cleaved to form a soluble trimeric molecule.2 BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells.
Product Description
CHO CellsRecombinant hCD269(BCMA)-muIg is a recombinant human fusion protein produced in CHO Cells cells. Molecular Structure: A soluble molecule consisting of the murine CD8 alpha signal peptide( 10 amino acids), extracellular (51aa) domain of human BCMA fused to the murine IgG2a Fc (235 aa).
Alternative names: CD269-muIg, CD269-Fc, BCMA-Fc, BCMA-muIg
Protein Specifications
| Fusion Format | Murine IgG Fc Fusion (muIg) |
|---|---|
| Expression System | CHO Cells |
| Molecular Weight | 33 kDa |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Species Reactivity | Human |
| Validated Applications | ELISA |
| Available Conjugates | Biotin, Unconjugated |
✔ Research Use Only (RUO)
Functional Activity
BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule’s abiltiy to bind to receptors on Raji cells. It has been used in vitro to block binding of recombinant BAFF (6) and function of APRIL (7).
Safety & Handling
Research Use Only. Not intended for diagnostic, therapeutic, or clinical use. Handle according to good laboratory practice (GLP). Consult the Safety Data Sheet (SDS) prior to use.
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This protein was expressed in CHO Cells cells. Mammalian expression is critical for this Fc fusion protein because it ensures proper glycosylation and tertiary folding of the extracellular immunoglobulin-like domains, preserving native receptor-binding activity.
Recombinant hCD269(BCMA)-muIg has been validated for: ELISA. It is produced as a Murine IgG Fc Fusion (muIg), making it suitable as a blocking reagent, binding standard, or functional stimulant/inhibitor in immunology assays.
Recommended storage: Store at 2 - 5°C. Freeze/Thawing is not recommended.. Formulation: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate. Avoid repeated freeze-thaw cycles. If long-term storage is needed, aliquot upon first use.
Species reactivity includes: human. Cross-reactivity was confirmed by functional binding assays (e.g., ELISA and/or FACS) against cells or recombinant proteins from the indicated species.
Available formats include: Biotin, Unconjugated. Conjugated forms are ready-to-use without secondary detection reagents for flow cytometry. Unconjugated (purified) forms can be used with anti-Fc secondary antibodies in ELISA.
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- Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189 (11):1747-1756.
- Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.
- Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.
- Xia, X., H. Hsu, et al. 2000, J Exp Med , 192(1): 137-143.
- Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.
- Xu W, A Cerutti, et al. (2007) Nat Immunol 8(3): 294-303. PMID: 17259987.
- Bing He, Andrea Cerutti, et al. (2007) Immunity 26(6): 812-826. https://doi.org/10.1016/j.immuni.2007.04.014
