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Scientific Background
The p75 low affinity human Nerve Growth Factor receptor CD271(NGRF, TNFRSF16) is expressed on a variety of tissue types including a subset of splenic and nodal lymphocytes. Presence of this receptor supports uptake of intracellular calcium, but not mobilization (3). CD271 is a stem cell marker for hematopoietic(5,6,7), stromal (8) as well as cancer cells (9,10). CD271 has been reported to inhibit differentiation of mesenchymal cells into various lineages(11).
Product Description
CHO CellsRecombinant hCD271(NGFR)-muIg is a recombinant human fusion protein produced in CHO Cells cells. Molecular Structure: A soluble fusion protein consisting of the extracellular (195aa) domain of human NGFR fused to murine IgG2a Fc (233aa). N-teminal sequence: (29)KEACP
Protein Specifications
| Fusion Format | Murine IgG Fc Fusion (muIg) |
|---|---|
| Expression System | CHO Cells |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Species Reactivity | Human |
| Validated Applications | ELISA |
| Available Conjugates | Biotin, Unconjugated |
✔ Research Use Only (RUO)
Functional Activity
NGFR-muIg binds to coated NGFbeta in ELISA.
Safety & Handling
Research Use Only. Not intended for diagnostic, therapeutic, or clinical use. Handle according to good laboratory practice (GLP). Consult the Safety Data Sheet (SDS) prior to use.
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This protein was expressed in CHO Cells cells. Mammalian expression is critical for this Fc fusion protein because it ensures proper glycosylation and tertiary folding of the extracellular immunoglobulin-like domains, preserving native receptor-binding activity.
Recombinant hCD271(NGFR)-muIg has been validated for: ELISA. It is produced as a Murine IgG Fc Fusion (muIg), making it suitable as a blocking reagent, binding standard, or functional stimulant/inhibitor in immunology assays.
Recommended storage: Store at 2 - 5°C. Freeze/Thawing is not recommended.. Formulation: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate. Avoid repeated freeze-thaw cycles. If long-term storage is needed, aliquot upon first use.
Species reactivity includes: human. Cross-reactivity was confirmed by functional binding assays (e.g., ELISA and/or FACS) against cells or recombinant proteins from the indicated species.
Available formats include: Biotin, Unconjugated. Conjugated forms are ready-to-use without secondary detection reagents for flow cytometry. Unconjugated (purified) forms can be used with anti-Fc secondary antibodies in ELISA.
Can't Find What You're Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
- Brodie C, E W Gelfand (1992) Journal of Immunology. 148(11): 3492-3497.
- Fantini F, O. Johansson (1992) Journal of Investigative Dermatology. 99(6): 734-742.
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- Kuçi S, Bader P, et al. (2010) Haematologica. 95(4):651-9.
- Hermida-Gómez T, Blanco FJ, et al. (2011) Tissue Eng Part A. 17(7-8):1169-79. 8) Jones E, McGonagle D, et al. (2010) Arthritis Rheum. 62(7):1944-54.
- Boiko AD, Weissman IL. Et al. (2010) Nature 466(7302):133-7.
- Civenni G, Sommer L, et al. (2011) Cancer Res. 71(8):3098-109.
- Mikami Y, Honda MJ, et al. (2011) Stem Cells Dev. 20(5):901-13.
