| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Recombinant His Tag |
| Clonality | |
| Host | |
| Immunogen | Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this His Tag antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target |
Overview
His Tag antibody supplied as a purified reagent for WB, IP, ICC, FACS, IHC in All Species samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.
Key elements and design rationale
- Antibody identity: Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM146.
- Format and purification: format: Purified; purity: Protein A purified from animal origin-free supernatant.
- Species reactivity (reported): All Species.
- Applications (listed): WB, IP, ICC, FACS, IHC.
- Immunogen / epitope context: Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this His Tag antibody..
These attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.
Biological background
His Tag is the intended antigen for this primary antibody. Reported biological context includes: This His Tag antibody reacts to proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Research relevance and current trends
- Spatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.
- Interaction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.
Common research applications
- Western blot (WB): compare relative abundance/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.
- Immunoprecipitation (IP): enrich native target and associated complexes for downstream analysis (e.g., immunoblotting or mass spectrometry) and interaction mapping.
- Immunocytochemistry (ICC): visualize intracellular distribution and morphology-linked changes in cultured cells.
- FACS: commonly used to measure relative target levels or localization changes in the context of the experimental question.
Across these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.
Notes for experimental interpretation
- Isoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.
- Species differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.
- Control concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout/knockdown/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.
Epitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
