| Field | Specification |
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| Alternative Names | 180 kDa Sin3-associated polypeptide (Sin3-associated polypeptide p180) (Breast cancer-associated antigen BRCAA1) (Histone deacetylase complex subunit SAP180) (Retinoblastoma-binding protein 1-like 1) (ARID domain-containing protein 4B) (BRCAA1) (RBBP1L1) (RBP1L1) (SAP180) |
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| Form | Liquid or Lyophilized powder |
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| Reconstitution | |
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Overview
This Recombinant Protein provides recombinant ARID4B from Homo sapiens (Human), produced in E.coli (region 167-415aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).
Key elements and design rationale
- Region: 167-415aa (domain boundaries can affect binding/activity readouts).
- Expression host: E.coli (may differ from native PTMs/processing).
- Tag(s): His (supports purification/detection; consider tag effects in controls).
Biological background
Also reported as 180 kDa Sin3-associated polypeptide (Sin3-associated polypeptide p180) (Breast cancer-associated antigen BRCAA1) (Histone deacetylase complex subunit SAP180) (Retinoblastoma-binding protein 1-like 1) (ARID domain-containing protein 4B) (BRCAA1) (RBBP1L1) (RBP1L1) (SAP180). Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor.
Research relevance and current trends
- Use of recombinant standards to improve assay calibration and cross-study comparability.
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor.
Common research applications
- Standard curve or spike-in reference for quantitative assays involving ARID4B
- Binding and specificity benchmarking for detection reagents (conceptual)
Notes for experimental interpretation
- Recombinant constructs may not capture all native isoforms or PTMs.
- Consider tag- or host-related effects when interpreting binding or activity.
- Use appropriate blanks and matrix/control concepts to separate signal from background.
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