| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Alternative Names | (JIP-4)(JNK-interacting protein 4)(Cancer/testis antigen 89)(CT89)(Human lung cancer oncogene 6 protein)(HLC-6)(JNK-associated leucine-zipper protein)(JLP)(Mitogen-activated protein kinase 8-interacting protein 4)(Proliferation-inducing protein 6)(Protein highly expressed in testis)(PHET)(Sperm surface protein)(Sperm-associated antigen 9)(Sperm-specific protein)(Sunday driver 1) |
| Conjugate | |
| Endotoxin Level | |
| Expression System | |
| Form | Liquid or Lyophilized powder |
| Molecular Weight | |
| Product Type | |
| Protein Length | |
| Purity | |
| Reconstitution | |
| Species | |
| Storage | |
| Target | |
| UniProt # |
Overview
Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial is a recombinant protein preparation from Homo sapiens (Human) designed for use in assay development, binding studies, and functional characterization. Key attributes such as expression system, expressed region, and affinity tag(s) help researchers match the reagent to specific experimental readouts.
Key elements and design rationale
- Expression system: E.coli expression is commonly used for rapid, scalable production. For targets that require glycosylation or other post-translational modifications, consider how a prokaryotic system may affect folding or activity.
- Expression region: The expressed fragment (408-534aa) focuses the reagent on a defined domain/segment, which can influence binding interfaces and epitope availability.
- Tag(s)/format: His/Myc tags can support purification and detection in pull-down or binding assays; confirm that the tag position does not interfere with the interaction of interest.
- Purity: ≥85% (SDS-PAGE) provides a quick checkpoint for reagent quality in downstream analytical workflows.
- Form: Supplied as Liquid or Lyophilized powder; select the format that best fits your lab’s handling and aliquoting preferences.
Recombinant design choices (expression host, fragment boundaries, and tag configuration) help balance yield, solubility, and assay compatibility. Choose conditions and controls that match the recombinant format to your experimental question.
Biological background
SPAG9 has been reported to be involved in The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex. Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking.. When interpreting results, consider species context, domain architecture, and whether the recombinant format represents full-length or a defined region.
Research relevance and current trends
- Increasing use of recombinant proteins as standardized reagents for cross-study comparability in quantitative assays.
- Structure-guided design of domain fragments to dissect binding interfaces and functional regions.
Common research applications
- Binding and interaction assays: quantify partner binding and rank conditions using plate-based formats or biophysical methods (SPR/BLI).
- Enzymology: assess catalytic activity and compare substrate preferences or inhibitor effects using appropriate controls.
- Assay development: use as a standard, spike-in control, or positive control where consistent specifications are required.
Interpretation typically relies on relative comparisons (treated vs control, mutant vs wild-type, or dose/time series) using consistent sample handling and appropriate normalization.
Notes for experimental interpretation
- Post-translational modifications: expression system can affect glycosylation and processing; interpret differences cautiously when comparing to native protein.
- Isoforms and domains: expressed regions may not capture all isoform-specific features; match fragment boundaries to your assay’s binding site.
- Controls: include blank matrix controls, tag-only controls (where relevant), and orthogonal readouts (e.g., WB/qPCR/ELISA) to support interpretation.
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
