| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chromodomain Helicase DNA binding protein 4, Mi-2b, Mi2-BETA, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, EC 3.6.4.12, EC 3.6.1. |
| Cellular Localization | |
| Expression System | |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Sf9 insect cells. |
| Species | |
| Storage | |
| Target |
Recombinant Human Chromodomain Helicase DNA Binding Protein 4 is supplied as a recombinant protein for in vitro research use.
Background
CHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
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Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. - Coating concentration: 0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
What is the purity of Recombinant Human Chromodomain Helicase DNA Binding Protein 4 (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Chromodomain Helicase DNA Binding Protein 4 (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
